N. H. SwellenctRP]bel 
After the aggregation is completed we note that the nuclei of the 
trophozoites are no longer irregularly disseminated throughout the 
protoplasm but are arranged in pairs. This arrangement becomes 
gradually more apparent during the earlier stages of sporulation ; it 
is caused by the daughter nuclei adhering to each other after nuclear 
division (Diagram IX). This division begins during and after en- 
cystment*. 
Diagram VIII. (Magnification of Diagram I.) Large cyst containing pansporoblasts with 
pairs of nuclei and spores. Only the outlines of the spores are drawn. In the inferior 
part of the cyst some elements of connective tissue are to be seen. 
1 It must be remembered that the diagrams only show small cysts (except Diagram XI); 
this is for convenience sake because drawings of large cysts would take too much place. 
There is, however, no reason to suppose that sporulation in large cysts is performed in a 
way different from that followed in small cysts. 
