146 
Spirochaetae and Acanthia 
Experiment V (a), at 24° C. 
Bugs fed on 
2. iv. 1907 
27. v. 1907 
Time when bug Appearances of spirochaetes in gut-contents 
was examined ,,-■'- 
after feeding Fresh Stained 
34 hrs. 
Most spir. fairly active, 
some sluggish 
6 „ 
Very slight intermittent 
motion, some mo¬ 
tionless 
8 „ 
Motionless, some short, 
others with globular 
swelling. 
22 „ 
Motionless 
31 „ 
None found 
46 „ 
>* »J 
72 „ 
Not examined 
96 „ 
>> JJ 
Experiment V ( b), at 
6,8, 24,48,96 hrs. Practically same result as 
Exp. V: motionless 
after 8 hrs., no spir. 
after 48 hrs. 
Mostly spirals, stain well, a few beaded 
and dividing; r. b. c. vanishing. 
Many rigid spirals, a few pale-staining, 
others with one half thick and frag¬ 
mented chromatin; r. b. c. vanishing. 
Ditto. 
Many, some normal, others slender or 
pale and fragmented, no r. b. c. 
Ditto. 
Only 2 found, faintly stained, no r. b. c. 
One bunch of spir. seen, fairly stained. 
None found. 
24° C. 
6 hrs., spiral and wormlike forms, these 
and r.b. c. stain well; 8—24 hrs. ditto, 
r. b. c. vanishing; 48—96 hrs., none 
found, no r. b. c. 
Summary of the foregoing experiments. 
It will be seen from the foregoing protocols of the experiments that 
the spirochaetes still showed slight movement after 
139 hours at 12° C. and were fairly active after 95 hours. 
122 „ 14° C. 
42 ,, 16° C. and were fairly active after 28 hours. 
6 „ 20° C. 
6 „ 24° C. and were fairly active after 34 hours. 
In stained films the spirochaetes mostly appeared normal up to 
278 hours at 12° C. 
213 ,, 14° C. 
19 ,, 16° C. though some were thick and beaded or irregular. 
6 ,, 20° C. ,, ,, ,, ,, 
3| „ 24° C. 
No traces of spirochaetes could be detected in stained films after 
139 hours at 20° C. 
96 hours at 24° C. 
Some dividing forms were seen after 
8| hours at 20° C. 
H „ 24° C. 
