162 Haemapliysalis punctata 
to Neumann, H. punctata is sufficiently cosmopolitan but does not 
appear with frequency; the specimens collected are never very numerous. 
Canestrini and Berlese found nymph and larvae in Italy, on Lacerta 
viridis and in Dalmatia on Lacerta muralis var. pelagosae. Neumann’s 
specimens came from a lizard in the Island of Cyprus, and from horse, 
hare, red partridge and grey partridge (Aveyron). 
Since Neumann’s description appeared, H. punctata has again been 
recorded in England by Pocock (1900, p. 326), who states that the 
specimens were collected by Mr F. Pickard-Cambridge at Dungeness, 
on a hedgehog and amongst shingle on the beach. Neumann (1901, 
p. 260) records 11 5j? ’s, in 5 lots, of which one lot is at the Hamburg 
Museum and four at the Berlin Museum, which were derived from 
Athens, Crete, the Cyclades, Teneriffe and Japan : 9 nymphs found on 
Lacerta ocellata var. tingitana, were collected by Domergue at Djebel 
Ksel in Algeria: 3 nymphs were found on Viper a aspis by C. Parona at 
Genoa; 1 $ found on the hedgehog by Oudemans in Holland. 
In the course of the last few years, we have received large 
numbers of specimens from Kent, especially the districts surrounding 
Lydd and Canterbury: the great majority of these were taken from 
sheep, but specimens taken from goats and ferrets have been received. 
Technique and Methods. 
In the study of the external anatomy of Haemapliysalis punctata, 
the authors have examined living specimens; killed, cleared and mounted 
entire in various media; dissections and sections. The coloured figures 
are faithful reproductions of living specimens, the original drawings of 
which were made by means of the Abbe camera lucida and amplified by 
numerous sketches of mounted specimens. 
The Zeiss binocular dissecting microscope has proved itself of the 
greatest value in the making of fine dissections and in the execution of 
sketches of the living tick. 
Specimens intended for the study of the structure of the chitinous 
exoskeleton were usually treated with a cold 10 °/ 0 solution of 
potash for some hours, or until the soft parts were entirely dissolved, 
washed in distilled water containing a trace of acetic acid, stained with 
picric acid, orange G or osmic acid (1% aqueous solution), dehydrated 
by transference through graded alcohols, cleared in xylol or oil of cloves 
and mounted in Canada balsam. It was found that the chitin was 
efficiently stained by immersion for one minute in xylol saturated with 
