28 
Growth of Bacilli 
twisted. Figs. 2 and 3 (25 and 35 minutes) show the further 
development of the loops. In Fig. 4 (45 minutes) continued develop¬ 
ment has resulted in the formation of a loose tangle. The knot resulting 
from the further development of the tangle is seen in Fig. 5 
(117 minutes). The gradual intertwining of the thread composing 
the tangle results in the formation of a tight knot at first slightly 
smaller than the original tangle. The two twisted loops, A and B, seen 
in Fig. 1 have developed into twisted processes, Aj and B^ Two 
other lateral processes C and D arising directly from the original chain 
are also seen. 
A 
Diagram 2. Illustrating the development of B. anthracis in the depth of agar. 
The lateral outgrowths of the deep colonies of B. anthracis are all 
due to such spiral or twisted outgrowths from the chain. These 
processes occasionally develop tangles or knots at various places in their 
length. In very rare cases only has a complete rupture of the original 
chain been seen. 
Plate IV, Fig. 11 illustrates a typical deep anthrax colony in agar. 
It consists of a dense central mass with numerous more or less radial 
