Gr. H. Drew 
My thanks are due to the Marine Biological Association of the 
United Kingdom for their kindness in granting me a table at their 
Plymouth Laboratory. 
Glugea shiplei, a new Sporozoon, parasitic in the muscles 
of Gadus luscus. 
A small specimen of Gadus luscus, caught at Plymouth, was seen to 
have a number of oval translucent cysts irregularly scattered all over 
the muscles of the body. The fish only measured 10 centimetres in 
length, and was so small that on holding it up to the light, besides those 
cysts which were visible from their comparatively superficial position, a 
number of cysts could be seen deeply embedded in the muscles of 
the posterior part of the body. 
The largest cysts averaged 5 mm. in length by 3 mm. in breadth, and 
contained a colourless gelatinous substance, in which one or more 
opaque white spots could be seen with the naked eye: the cyst wall 
was very thin and transparent. The cysts were present not only in the 
skeletal muscles, but also in those of the stomach and intestinal walls. 
Only muscular tissue was infected, and no trace of cysts could be found 
in any other tissue. The superficial cysts were not connected with the 
skin, and none had ruptured externally. 
Some of the cysts were cut into, and the somewhat gelatinous 
contents turned out on a slide : under low powers of the microscope the 
jelly-like substance was seen to contain a number of circular bodies 
resembling nuclei, and the white opaque body appeared to consist of a 
dense mass of minute spores. On teasing out these spores, and 
examining under high powers, the spores were seen to be pyriform in 
shape, measuring 3'5/z. long, by 2'5yu. at their broadest axis (fig. 2). A 
small vacuole was pi’esent at the broader end, and a very minute body 
at the apex probably represents the polar capsule. In none of the 
spores could any filament be distinguished ; attempts to cause protrusion 
of a filament by treatment with reagents were unsuccessful. 
The shell of the spore was resistant to the action of strong sulphuric 
acid, and the dehiscence of the valves could not be determined. 
The vacuole was auiodinophilous. Portions of the muscular tissue of 
the ti’unk, containing these cysts, were excised and fixed in various 
fluids : somewhat unexpectedly those fixed in 5 °/ 0 (of 30 °/ 0 ) formol 
alcohol gave the most satisfactory results. The tissue was embedded in 
