Gr. H. F. Nuttall 
111 
toplasm. The nucleus is usually of rounded or oval form with a sharply 
defined contour. In some nuclei, fine chromatin particles appeared to 
be aggregated at the periphery or to be scattered through the pale pink- 
staining substance of the chromatic nucleus. When nuclear division 
occurs (see below under Mode of Multiplication) the chromatin appears 
to be more diffused and the nucleus is of irregular form. In one case 
the extrusion of what may have been a dividing nucleus was observed 
(Fig. 15). In this case the chromatin is peripherally distributed as 
though surrounding a vacuole, but the appearance may be unnatural, 
especially as the blue-staining parasite itself appears “blistered,” as is 
occasionally seen in Piroplasma in imperfectly stained films. 
Mode of Multiplication. The manner in which the parasite under¬ 
goes division within the corpuscle appears to be as follows: when a 
single parasite (PI. X, Figs. 1—4) has attained a certain size, its nucleus 
divides (Figs. 5—6) and this is followed by division of the parasite into 
two (Figs. 7—12). The newly-formed pair of parasites, if sufficiently 
mature, may repeat the process (Figs. 15—19), thus giving rise to four 
parasites within a corpuscle (Figs. 20—22). Nuclear division is simple 
and clearly precedes the division of the protoplasm both in single para¬ 
sites (Figs. 5 —6) and in pairs of intracorpuscular parasites (Figs. 15—19). 
In parasites about to divide the chromatin of the nucleus appears to 
be diffuse (Fig. 4) or drawn out in an irregular thread-like manner 
(Figs. 13, 14) as if the nucleus were in a state of flux. The chromatin 
becomes aggregated at opposite ends of the parasite and the connecting 
strand ruptures (Fig. 15), after which the newly-formed nuclei become 
rounded (Figs. 15—19). 
In view of the small number of parasitized corpuscles which could be 
discovered in the film it is impossible to say if more than four parasites 
may be formed in the corpuscle. Judging, however, from the absence of 
smaller forms than those figures (Figs. 18, 23, 29) it is reasonable to 
assume that not more than four parasites are formed within a cor¬ 
puscle. 
Enumeration of the Types of Parasites encountered. 
Intracorpuscular parasites. The complete examination of the smears 
from the jackal only revealed 90 parasites. Sketches and notes were 
made of all the parasites encountered. In ordering the data thus collected 
it was found that the majority of the infected corpuscles (81 °/o) con¬ 
tained one, two or four discrete parasites, whereas the remaining 
