232 
Rabies 
was removed from the sections, which were then stained with Mallory’s 
Iron haematoxylin and Bordeaux Red. 
In each monkey, 100 ganglion cells were examined and the size, 
number, and colouration of the cell inclusions, noted and recorded. 
The result of this examination is shown in Table IV, which gives their 
frequency distribution in size etc. 
Our results may be summarized as follows:— 
1. With subpassage, Negri bodies diminish in size until they 
become almost ultra-microscopic. 
2. Of large size to begin with and staining brown to black, in the 
above experiment by the 6th passage they stain wholly black and are 
identical in staining reactions with the nucleolus. 
3. There is a very decided drop in the numbers present at the 
7th passage. The significance of this is not exactly clear. It is note¬ 
worthy, however, that the 7th passage is that in which the paralytic 
form of rabies is fully developed. In the 6th passage furious symptoms 
were still marked. 
4. The diminution in the number of Negri bodies shown in the 
7th passage, is continued in the 8th and following passages. 
5. The Negri bodies of the earlier passages (first three) showed a 
“ veil ” arrangement. Those of the later passages did not present this 
appearance and may be described as “ naked.” 
6. We regard the granules found in “veiled” Negri bodies as 
being composed of unaltered nucleolar substance. 
7. The experiment given seems to show that the Negri body, as 
found in the cytoplasm of a ganglion cell, tends to disappear altogether 
from that region with the attainment of fixity of the virus. We have 
inoculated bullocks—the animals showing the largest Negri bodies 
known to us—one with street virus and another with fixed virus. The 
street virus brain showed large Negri bodies; the fixed virus brain 
showed none at all. 
