A. C. Coles 
251 
straight, the posterior thrown into two slight curves. It stains a pale 
red colour with Giemsa. The margins are surrounded by granules, 
which are arranged somewhat loosely, giving the impression, so often 
seen in the trypanosomes of birds, of being surrounded by a lighter 
stained area. No definite internal structure can be made out. 
The kinetonucleus is a very insignificant looking structure by no 
means easily seen. It is round, deeply stained, and lies in an area 
in which there are fewer granules. It is situated about 5 /r from the 
centre of the nucleus, and has a diameter of about 1'2 fi. 
It is by no means easy to trace the attached portion of the flagellum 
directly to the kinetonucleus, although there are areas of fewer granules 
along its path. 
The distorted trypanosome differs considerably in its general ap¬ 
pearance from that described above. As will be seen from the figure, it 
is a large stumpy form, but during the preparation of the film its 
posterior extremity has been crushed and therefore it is impossible to 
give any accurate description. In this individual the flagellum does not 
seem to run up to the kinetonucleus but stops a short distance in front 
of it, the interval being made up by an exceedingly fine line which can 
be recognised in the photograph. 
The dimensions of the perfect trypanosome are as follows: total 
length, 98/r; length of body, 88/r; free flagellum, 10 ; distance from 
posterior extremity to kinetonucleus 37 fi, and to the centre of tropho- 
nucleus, 44 /x ; distance between trophonucleus and kinetonucleus, 5'5 /a ; 
diameter of body at trophonucleus, 6 ya, together with the undulating 
membrane, 9‘5 ya. 
In conclusion I should like to express my thanks to Mr J. W. Ogilvy 
and Mr Wm. Harding for their kindness in taking the microphotographs. 
REFERENCES. 
Behn, P. (1910). Ueber Entwicklungsformeu des Trypanosoma franki. Berliner 
tierdrztliche Wochenschnft^ xxvi, 809-810. 
Crawley, H. (1912). The Protozoan Parasites of Domesticated Animals. Biireau 
of Animal Industry, U.S. Department of Agriculture. Report No. 27 (for the 
year 1910), pp. 184-187. 
Frank, G. (1909). Ueber den Befund von Trypanosomen bei einem in Stein-Wingert 
(Westerwald, Regierungsbezirk Wiesbaden) verendeten Rinde. Zeitschr. f. 
Infektionskrankh. paras. Krankh. u. Hyg. d. Haustiere, v, 313-315. 
Knuth, P. and Rauchbaar, G. (1910). Zum Vorkommen von Trypanosomen bei 
Rindern in Deutschland. Berl. tierurztl. Wochenschr. xxvi, 609. 
