C. Strickland 
85 
Rat No. 
Hours of fast 
No. of lice 
Result 
54 
20 
1 
— 
*69 
18 
1 
+ very active 
*70 
24 
1 
+ 
*71 
24 
1 
- 
89 
24 
8 
+ in 4 
90 
48 
3 
+ in 1 
6 
15 
+ in 7 
* signifies not recorded under section (B). 
Thus 15 lice were examined after a fast of 18—48 hours, and in 7 
T. lewisi still persisted, even in one case being very active after the louse 
had fasted 48 hours. No developmental changes were observed to occur 
in the trypanosomes. 
D. The influence of digestion in lice on T. lewisi. 
A number of observations were made on the state of the digestive 
process in the lice, as evidenced by the colour of the gut-contents, with 
a view to seeing if the persistency of the T. lewisi was in any way related 
to it. There did not appear to be any definite relation, for sometimes 
when the stomach of the louse contained bright red blood no trypano¬ 
somes could be found, whilst at other times, when the gut-contents 
shewed the last stages of digestion, trypanosomes were found. 
The following Table gives the results of the observations made. 
Louse 
Colour of 
Louse 
Colour of 
Rat No. 
No. 
gut-contents 
Result 
Rat No. 
No. 
gut-contents 
Result 
18 
1 
b. red 
+ 
57 
19 
brown 
- 
2 
d. brown 
+ 
20 
b. red 
+ 
40 
3 
b. red 
- 
21 
b. red 
- 
4 
b. red 
- 
22 
d. red 
- 
52 
5 
pale yellow 
- 
23 
d. red 
- 
54 
6 
b. red 
+ 
64 
24 
d. red 
+ 
7 
d. red 
- 
25 
brown-green 
+ 
8 
red-brown 
- 
26 
b. red 
+ 
57 
9 
d. red 
+ 
65 
27 
straw 
+ 
10 
d. red 
+ 
28 
b. red 
+ 
11 
b. red 
+ 
29 
b. red 
+ 
12 
b. red 
+ 
30 
d. brown 
— 
13 
brown 
+ 
31 
d. brown 
14 
b. red 
+ 
32 
b. red 
+ 
15 
b. red 
+ 
33 
straw 
- 
16 
red-brown 
- 
34 
b. red 
+ 
17 
red-brown 
- 
35 
b. red 
+ 
18 
brown 
- 
36 
b. red 
+ 
b. = 
bright. 
d. - dark. 
