G. H. F. Nutt all and S. Hadwen 
175 
Experiments with Trypanblau. 
Owing to the fact that Trypanblau appeared to exert a more marked 
effect upon the parasites than did Trypaurot, we conducted the rest 
of our experiments with Trypanblau. Of the 10 dogs which underwent 
curative treatment, seven made a good recovery. Three of these dogs 
are still alive (17. VI. 09), having been respectively under observation 
for periods of 90, 83 and 65 days. Four of the recovered dogs subse¬ 
quently died of intercurrent disease (distemper mostly, and mange) 
having lived respectively for 42, 43, 52 and 69 days after they had been 
inoculated. Three of the treated dogs died of piroplasmosis, namely, 
a dog (12) treated on the day of its death and which was to be 
regarded as a hopeless case at the start, and dogs 10 and 11, which 
died of a relapse. The last-named dogs were very poor subjects for 
experiment, as will be seen by reference to the protocols. 
Finally, one dog (13) received a dose of the drug before the parasites 
would naturally have appeared, that is, 24 hours after inoculation with 
virulent blood. This dog never showed parasites in its blood, and it 
remained perfectly healthy up to the 65th day after inoculation. 
On the other hand, all of the dogs (7) which served as controls for these 
experiments, died of piroplasmosis. 
The dogs which received curative treatment were as follows : 
Dog 
o/o of infected 
r.b.c. at time 
No. of doses Result of 
No. 
of treatment 
of drug given treatment 
No. Control dogs 
3 
0-2 
i 
Living 90th day ‘ 
‘ IV ” died 7th day. 
4 
3*5 
i 
,, 83rd ,, ‘ 
‘VIII” died 7th day. 
5 
0-4 
i 
Lived 69 days, when it died of ‘ 
distemper and mange 
‘ V ” died 9th day. 
6 
0-6 
i 
Living 65th day ‘ 
‘ VI ” died 8th day. 
7 
0 025 
2 
Lived 42 days, when it died of ‘ 
distemper 
‘ VII ” died 36th day. 
8 
? 
4 
Lived 43 days, when it died of ‘ 
distemper 
‘ I ” died 8th day. 
9 
•? 
2 
Lived 52 days, when it died ‘ 
from mange 
‘ IV” died 7th day. 
10 
4-5 
2 
Died 13th day “ 
piroplasmosis relapse 
1 VIII ” died 7th day. 
11 
3-8 
2 
Died 15th day 
piroplasmosis relapse 
5J 5 J >> 
12 
50 
1 
Died 3 hours after treatment, but parasites 
visibly affected by drug. 
Note : 
(Nos. 10, 
—Whereas all the (7) controls died of piroplasmosis only 3 of the 10 treated dogs 
11, 12) died from this cause. 
