186 
The Cure of Piroplasmosis 
Note to Dogs 10 and 11. 
Puppies 10 and 11 did not at first appear to suffer at all from the effects of the 
disease, and we expected them to recover, although they were wretched subjects for 
experiment, both weighing less than 2 lbs. They were undersized, thin, pot-bellied 
and wormy. 
In the case of Dog 10, on day 4 at 7 p.m., the percentage of infected red blood 
corpuscles appears very high (7 °/ 0 ), owing, no doubt, to the massage of the ear resorted 
to to promote the flow of blood to the punctured earvein. The high percentage of 
infected red blood corpuscles noted in the case of Dog 11 after treatment in two of the 
counts (indicated by *) may be attributed to the same cause. 
In the case of Dog 10, on day 5 at 1 p.m., over 3000 uninfected corpuscles were 
counted in the middle line of the film, and infected corpuscles (2 °/ 0 ) were only encountered 
at the edge of the film; at 10 p.m. only a very few parasites were found at the edge of the 
film, and subsequently none were encountered in this situation. On day 10 the parasites 
reappeared after an interval of four days. The dog started to have diarrhoea and passed a 
large Ascaris. The parasites subsequently increased in numbers, the infected corpuscles 
numbering 4 °/ 0 on the following day. The puppy, as noted above, was a very poor subject 
for experiment. On day 12 the treatment was without effect. The dog died on day 13, 
and its intestines were found to contain many tapeworms. 
In the case of Dog 11, the parasites, after being absent for five days, reappeared in 
small numbers in the peripheral circulation, the number of infected red blood corpuscles 
being moderate (2 °/ 0 ) on the day before it died. In Dog 10 no less than 4 °/ 0 of the red 
blood corpuscles were infected for two days preceding death. 
The blood counts made on the last days are remarkable as showing the active multipli¬ 
cation of the parasites as evidenced by the high percentage of dividing forms (D). That 
rapid invasion of fresh corpuscles was proceeding is also shown by the high percentage of 
single pyriform parasites (P) which occurred in the corpuscles. These observations possess 
an added interest, since they fully confirm the view maintained by Nuttall and Graham- 
Smith with regard to the mode of multiplication of P. canis in the circulating blood. 
(Compare also with Control Dog VIII, p. 171.) 
Dog 12. 
Fox terrier puppy. Weight 3 lbs. 6 ozs. Received one dose of drug when very ill. 
(8. hi. 09.) 
Day 
1. Inoculated with 4 e.c. of heart blood. 
11. No parasites found to date. 
Inoculated again with 90 drops of citrated blood. 
14. Day 4 from second inoculation, found 3 (PP) in film. 
15. ,, 5 ,, ,, ,, ,, 1-6 °/ 0 of r.b.c. infected. 
16. ,, 6 ,, ,, ,, ,, 50 °/o of r.b.c. infected: they con¬ 
tained 57J °/ 0 (PP) and 32J °/ 0 (O) parasites. 
Haemoglobinuria. Dog very ill. 
At 11.45 a.m. Treatment: 6 c.c. of sat. sol. Trypanblau. 
At 2.45 p.m. The r.b.c. contained 224 °/ 0 (PP) and 77J °/ 0 (O) parasites. 
At 3 p.m. Dog died from piroplasmosis. 79 u /o °f i'-b.e. infected. 
