G. H. F. Nuttall and G. S. Graham-Smith 209 
experiments. It must, however, be remembered that Miyajima con¬ 
ducted his experiments in Japan, whereas we conducted ours in England 
with parasites conveyed to cattle by means of ticks which were imported 
from South Africa. There can, however, be no doubt as to the similarity 
of the parasites with which we were dealing. 
We have to report upon two carefully conducted experiments in 
which we failed to obtain results confirming those of Miyajima. 
Culture 
ed afti 
lowing 
of d 
elapse 
Blood 1 c.c., 
bouillon 2 c.c. to 10 c.c. 
Blood lc.c., '8 % NaCl, 
Solution 1 c.c. 
N. 
Blood 1 c.c., 
2 % citrate 
Solution 5 c.c. 
i. 
Corpuscles and Theileria normal. 
Same as in bouillon. 
— 
2. 
Corpuscles normal or crenated, 
some with normal Theileria. 
Large vacuolated granular leu¬ 
cocytes found in groups. 
Corpuscles and Theileria 
normal. 
3. 
Corpuscles normal or crenated; 
Theileria mostly marginal or 
rounded, slight dancing move¬ 
ment ; a few swollen leucocytes. 
When stained, some Theileria 
still take blue colour. Chro¬ 
matin in parasites aggregated in 
masses. 
Same as in bouillon. 
When stained, corpuscles 
appear shrunken, some 
parasites free, some mar¬ 
ginal. 
Same as in 
bouillon. 
4. 
Same as before. 
Same as before. 
Same as before. 
5. 
JJ 9 9 
9 9 9 9 
>> 
»> 
6. 
99 9 9 
9 9 9 9 
99 
9 9 
12. 
Corpuscles well preserved. Thei¬ 
leria mostly rounded and mar¬ 
ginal. When stained,corpuscles 
appear pale, Theileria at times 
free; stain intensely with Gi- 
emsa but without trace of blue. 
Corpuscles shrunken, 
slightly crenated; Thei¬ 
leria rounded, marginal. 
Stained appearances 
much the same as in 
bouillon. 
Same as in 
bouillon. 
Exp. I. In this experiment 30 ticks were placed on a cow on 
10. ii. 1909. On 6. ill. an examination of the blood showed that 56 °/o 
of the red blood-corpuscles harboured Th. parva. Blood was now taken 
from a vein with aseptic precautions, and, after it had been defibrinated 
by being shaken for 25 minutes in sterile glass-stoppered bottles, 
measured quantities of the blood were mixed with measured quantities 
of bouillon, sodium citrate or sodium chloride solutions respectively. 
The sterile cotton-plugged vessels, containing the mixtures, were then 
incubated at 24° C. The vessels used were (a) ordinary test-tubes, 
and (6) small bottles such as we had used successfully for the cultivation 
of Piroplcisma canis. The bouillon, which was approximately neutral, 
Parasitology ii 
14 
