G. H. F. Nutt all and G. S. Graham-Smith 219 
plasmosis with many parasites in their blood. The history of Dog 4 is 
given in considerable detail, the blood having been examined daily by 
Nuttall and Hadwen. Methylene blue was injected subcutaneously on 
the 8th day after inoculation, but the dye exerted no effect upon the 
parasites. On the 9th day 14 °/o °f the corpuscles were found to be 
infected. Subsequently the number of infected corpuscles decreased but 
the dog showed severe symptoms. Parasites could not be detected in 
blood films on days 15, 18, 19 and 21—28. The parasites then 
reappeared and the dog finally succumbed to the chronic form of piro- 
plasmosis. 
Inoculations with fluid blood containing dead parasites. 
Dog 1. 
(Puppy), received 8 subcutaneous injections of citrated blood obtained from three dogs 
■which died of piroplasmosis. The amounts of blood injected (deducting the citrate solution 
with which it was mixed) were as follows : 
Doses of blood in c.c. = 
Number of days the blood) 
had been kept in vitro } 
Intervals between injee-) 
tions, in days j 
5 2-5 2-5 5-3 2-2 
5 6 7 6 8 
2 2 10 2 2 
6 - 6 6 - 6 6’6 = Total 42 c.c. 
10 15 3 
5 5 
Ten days after the last injection the dog received 3 c.c. of virulent blood. 
Result: 10 days after inoculation a very few parasites were found in the dog’s blood 
and 8 days later (18 days after inoculation) the dog died during the night from no apparent 
cause. The dog had not shown haemoglobinuria and no parasites could be found in its 
heart-blood at autopsy. 
Dog 2. 
(Large Terrier), received 4 subcutaneous injections of concentrated suspensions of 
infected corpuscles (sedimented in citrate solution containing a few drops of carbolic acid). 
The amounts injected are reckoned in terms of ordinary blood. 
Doses of blood in c.c. = ... ... 10 10 4 15 = Total 39 c.c. 
Number of days the blood had been in vitro 8 10 12 15 
Intervals between injections, in days ... 2 2 3 
A week after the last injection the dog was inoculated with virulent blood. 
Result: The dog died of piroplasmosis after 10 days. 
Dog 3. 
(Terrier, adult) was treated like Dog 2. It received 5 injections; the amounts injected 
being reckoned in terms of ordinary blood. 
Doses of blood in c.c. = ... ... 10 18 20 18 20 = Total 86 c.c. 
Number of days the blood had been in vitro 8 9 12 8 10 
Intervals between injections, in days ... 13 3 3 2 
