G. H. F. Nuttall and G. S. Graiiam-Smith 221 
21 
104-0 
Dog stronger, no infected r.b.c. ... 
• 
22 
104-8 
»» »> >» >» >> ••• 
23 
102-2 
Blood more normal, no infected r.b.c. 
24 
101-4 
No infected r.b.c. 
25 
100-1 
»» »> >» 
26 
101-7 
No parasites 
27 
101-8 
>> ,, 
28 
103-6 
„ , 9 
29 
105-2 
Parasites reappear 
0-4 
2 
32 
34 
10 
18 
2 
2 
30 
105-4 
Dog ill. Blood count: 
a.m. (200 infected r.b.c. recorded) 
1 
• 
1 
22-5 
32 
4 
1-5 
0-5 28-5 
0-5 
9-5 
p.m., sudden fall of infected r.b.c., 
only 11 found on whole film- 
edge : 6 (O), 1 (PP), 4 (PPPP) 
+ 
• 
• 
+ 
+ 
# 
+ 
. 
# 
31 
104-8 
4 infected r.b.c. found on whole 
film-edge: 2(0), 1 (PP),1(PPPP)... 
+ 
• 
• 
+ 
+ 
• 
• 
• 
+ 
• 
• 
32 
102-4 
6 infected r.b.c. on film-edge: 
3 (O), 3 (PP) 
+ 
• 
• 
+ 
+ 
• 
• 
• 
• 
• 
• 
33 
104-2 
Parasites in larger numbers 
2 
5 
1 
31 
46 
2 
• 
. 
15 
• 
• 
34 
103-0 
n »> >> 
1 
7 
1 
37 
51 
• 
. 
. 
4 
. 
. 
35 
103-0 
>j >» »» 
0-5 
10 
. 
50 
36 
2 
. 
2 
• 
. 
• 
(20 v. 09) 
36 — Dog found dead. Death due to the chronic form of piroplasmosis. 
Note :—The signs P 4 , P 6 , P 8 indicate corpuscles which contained 4, 6, and 8 pyriform parasites 
respectively. 
Experiment with dried blood. 
The blood used for the preliminary treatment of the dog in the 
following experiment was dried in quantities of 10 c.c. for 5 days at 
35° C. The blood was subsequently kept at room temperature. For 
purposes of injection as much of the dried blood as possible was 
dissolved or brought into suspension in water by being rubbed up in a 
mortar or by being shaken up in sterile bottles containing glass beads. 
The grumous looking fluid was then injected subcutaneously. 
Dog 5. 
(Whippet) received 4 injections of blood solution. 
Doses of blood in c.c. = ... ... 8 8 8 8 = Total 32 c.c. 
Number of days the blood had been dried 6 9 11 13 
Intervals between injections, in days ... 3 2 2 
Nine days after the last injection the dog received 4 c.c. of virulent blood. 
Result: Seven days after inoculation parasites appeared in the dog’s blood and the 
animal subsequently died of piroplasmosis. 
(See Summary (2) on p. 227.) 
