230 
Treatment of Canine Piroplasmosis 
Whereas six of the dogs received a dose of Trypanblau on the day 
[“ 0 ”] before they were inoculated, one dog received the dose on the day 
after inoculation. All of the dogs were inoculated subcutaneously. The 
treated dogs were injected subcutaneously with the dye in cold saturated 
solution. 
Treated Dogs. 
Dog Day 
Dog 
Day 
1 
0 
Treatment: 2 c.c. of trypanblau 
4 
0 
Treatment: 2-3 c.c. of trypanblau 
solution. 
solution. 
1 
Inoculated with 3 c.c. of virulent 
1 
Inoculated with 3 e.c. of virulent 
blood. 
blood. 
22 
No parasites found to date, dog 
30 
No parasites found to date, dog 
well. 
well. 
2 
0 
Treatment: 2 c.c. of trypanblau 
5 
0 
Treatment: 2 c.c. of trypanblau 
solution. 
solution. 
1 
Inoculated with 3 c.c. of virulent 
1 
Inoculated with 3 c.c. of virulent 
blood. 
blood. 
26 
No parasites found to date, dog 
27 
No parasites found to date, dog 
well. 
well. 
3 
0 
Treatment: l - 8 c. c. of trypanblau 
6 
0 
Treatment: 2 c.c. of trypanblau 
solution. 
solution. 
1 
Inoculated with 3 c.c. of virulent 
1 
Inoculated with 3 c.c. of virulent 
blood. 
blood. 
23 
No parasites found to date, dog 
28 
No parasites found to date, dog 
well. 
well. 
Dog Day 
7 1 Inoculated with 5 e.c. of virulent blood. 
2 Treatment: 15 c.c. of trypanblau solution (large dog). 
28 No parasites found to date, dog well. 
Control Dogs. 
Dog 
Day 
A. 
1 
Inoculated with 3 c.c. of virulent blood. 
7 
Parasites appeared. 
10 
Dog died of piroplasmosis with many parasites in its blood. 
B. 
1 
Inoculated with 3 c.c. of virulent blood. 
23 
No parasites to date, dog well. 
As will be seen from the foregoing protocols, none of the seveu dogs 
which received Trypanblau developed piroplasmosis. At no time were 
parasites detected in their blood, although examinations were made up to 
the 22nd and to the 30th day after inoculation. On the other hand, one 
