G. H. F. Nuttall and S. Hadwen 
233 
It appears from the above experiments that Trypanblau, given by the 
mouth, is ineffective, and that it exerts no apparent influence upon the 
parasites. 
Experiments with Tryparosan. 
Owing to the excellent results recently obtained in the treatment of 
trypanosomiasis by means of Tryparosan in Ehrlich’s laboratory (Roehl, 
XII. 1908; Marks, v. 1909) it appeared to us desirable to try the effect 
of this dye upon piroplasmosis in dogs. Tryparosan is prepared from 
parafuchsin by the addition of chlorine to the molecule. The dye is 
prepared by Messrs L. Cassella & Co., Frankfurt a M. We are much 
indebted to this firm for their courtesy in supplying us with the dye 
free of cost for the purposes of our experiments and take this occasion to 
warmly thank Geheimrath Ehrlich for the help he has given us in 
procuring this and other remedies in connection with our investi¬ 
gations. 
In the following experiments both dogs (Irish terriers) were treated 
with Tryparosan. Dog 1 received a subcutaneous injection of a 1:500 
solution of the dye on the afternoon of the day when the parasites first 
appeared. Dog 2 was given the dye in capsules per os (a) on the day 
following the inoculation; ( b ) on the day before, and (c) on the day the 
parasites appeared. 
Dog 1. ( Subcutaneous Treatment.) 
Date 
Day 
Temp. 
“F. 
°/o Of 
infected 
r.b.c. 
(2. vii. 09.) 
I. 
— 
5.30 p.ru. Inoculated with 
4 c.e. of virulent blood. 
°/o 
(P) 
% 
(O) 
%> 
(PP) 
% 
(D) 
»/o 
(PPPP) 
'5. 
— 
11 a.m. Parasites appeared 
+ 
• 
• 
• 
• 
• 
— 
3.30 p.m. Treatment: 30 
c.c. of Tryparosan so¬ 
lution. Oedema soon 
followed at site of in¬ 
jection 
0-4 
1 
45 
44 
5 
6 
103-8 
5 p.m. Much oedema at 
site of injection 
1-0 
• 
43 
45 
3 
9 
104-4 
9.30 p.m. 
0-6 
1 
47 
44 
2 
6 
6. 
103-9 
11.30 a.m. 
15-0 
. 
45 
51 
3 
1 
104-1 
6.15 p.m. Dog looking ill. Much oedema. 
(8. vii. 09.) 
7. 
— 
9 a.m. Dog found dead. 
Death due to piroplasmosis. 
