234 
Treatment of Canine Piroplasmosis 
Date 
(2. vn. 09.) 
Dog 2. ( Treatment by the mouth.) 
Day 
1 . 
2 . 
3. 
4. 
Temp 
"F. 
6 . 
8 . 
(10. vii. 09.) 9. 
5.30 p.m. Inoculated with 4 c.c. of virulent blood. 
12.30 p.m. Treatment: gram. Tryparosan. 
11.30 a.m. Pink colouration of skin noticeable. 
— 3.30 p.m. Treatment. 
— 11 a.m. Parasites ap¬ 
peared : 2 (PP) found 
102-2 5p.m. Treatment: Jgram. 
Tryparosan. 
102-2 11.30 a.m. Few parasites 
102-4 6.15 p.m. Doglookswell. 
104-2 11 a.m. Dog looks well 
104-1 6 p.m. ,, ,, 
104-8 12.15 p.m. Dog looks 
fairly well ... 
— 9 a.m. Dog found dead. 
■tV gram. Tryparosan. 
°/o of 
infected 
r.b.c. 
°/q 
(P) 
°/o 
( 0 ) 
+ 
■05 
1-0 
18-0 
(Pf>) 
+ 
% %• % 
(D) (PPPP) (OO) 
40 40 
61 35 2 
52 37 2 
12 
Death due to piroplasmosis. 
Control Experiments. 
A Control Dog (Terrier) was inoculated at the same time as the four 
dogs referred to in the preceding two sets of protocols. The control dog 
received no treatment. 
Date 
(2. vii. 09.) 
(8. vii. 09.) 
Temp. 
Day ° F. 
1. 
.— 
5.30 p.m. 
Inoculated with 
4 c.c. of virulent blood. 
%Of 
infected % 
°/o 
(Pf) 
°/o 
% 
5. 
— 
11.30 a.m. 
A few parasites 
r.b.c. 
(P) 
(O) 
(D) 
(Pi) 
appeared 
+ 
• 
• 
• 
• 
• 
103-3 
5 p.m. 
6. 
103-9 
11.30 a.m. 
4 
2 
42 
47 
2 
6 
102-2 
6.15 p.m. 
Dog looks ill. 
°/o (P 5 ) 
to(P 8 ) 
7. 
95-2 
11a.m. Dog moribund ... 
88 
. 
23 
36 
• 
28 
14 
— 3 p.m. Dog died of piroplasmosis. 
Note: —The signs P 4 , P 5 , Pg indicate corpuscles which contained 4, 5 to 8 pyriform 
parasites respectively. 
It follows that Tryparosan exerts no influence upon Piroplasma 
canis and that it is ineffective as a remedy for canine piroplasmosis. 
Summary and Conclusions. 
1. Trypanblau injected subcutaneously into dogs a day before or a 
day after they have been inoculated with blood containing Piroplasma 
canis effectually prevents the development of piroplasmosis by destroying 
the parasites at the onset of infection. 
