266 
Drug Treatment of Redwater 
7. Although doses of 150—200 c.c. of a saturated watery solution 
of the dye were used, it is probable that smaller doses will prove 
efficient. The drug appears to produce no ill-effects upon cattle. 
8. The drug, being a dye, has the disadvantage of colouring the 
tissues, more especially the subcutaneous connective tissues. How 
long the colouration persists remains to be determined. In any case 
this disadvantage can scarcely weigh in the balance as against saving 
the life of the animal, especially when used for breeding purposes. 
9. We hope that experiments, which are about to be conducted in 
the field in Africa and elsewhere, will demonstrate the value of the 
remedy in practice. 
10. Trypanblau and similar drugs should be given a trial in the 
treatment of Cargeag in sheep and Biliary Fever in horses. 
Note regarding the solubility of Trypanblau. 
In the course of our experiments we noted that crystals of trypanblau were frequently 
deposited after a short time even in 1 °/ 0 solutions of the dye prepared with distilled water 
at room temperature. We therefore communicated with the manufacturers who had 
supplied us with the dye, Farbwerke vorm. Meister Lucius und Briining, Hoeehst a/M., 
desiring information regarding the solubility of trypanblau in distilled water. In a letter, 
dated 3 Aug. 1909, from the manufacturers, the latter state that they have made many 
experiments in this direction with the following results : 
100 c.c. boiling water will dissolve 2 grammes of trypanblau and the solution can be 
easily filtered. On the other hand 5 grammes of the dye will not easily dissolve in 100 c.c. 
of boiling water and the solution filters badly, part of the dissolved dye separating in the 
filter ; on cooling, the solution forms a gelatinous mass. The 2 °/ 0 solution, after stand¬ 
ing for 24 hours, has for the most part solidified into a gelatinous mass. In the case of 
1 °/ 0 solutions only a very small amount of the dye separates out after the lapse of 24 hours. 
A % solution remains quite clear after standing for a long time. Owing to the difficulty 
of obtaining solutions of the dye and the tendency of the solutions to gelatinize on stand¬ 
ing, it appears advisable to prepare the solutions immediately before use, in which ease a 
2 °/ 0 solution at blood temperature could be used. 
As stated we prepared our solutions in the cold, the dye being added in the proportion 
of 1 to 1'5 % to distilled water. Our solutions did not gelatinize, and there was always a 
certain amount of dye deposited. The deposit was fine and was mostly taken up 
in the syringe and injected with the solution. From a practical standpoint the 1 °/ # solu¬ 
tion appears to suffice and further experience may show that J°/ 0 solutions are sufficiently 
effective. G. H. F. N. 
