306 
Anticoagulin in Ticks 
used concentrated and diluted to various degrees so that £ to of active 
principle was contained in the '02 c.c. of saline which was mixed with 
an equal volume of blood. Result: 
Dilution 1 prevented clotting completely. 
„ ^ delayed clotting for 40 to 120 minutes. 
„ \ „ „ „ 30 minutes. 
Experiment III. The glands of 4 A. persicus (</, average length 
6'5 mm.) were emulsified in '05 c.c. of salt solution, the emulsion being 
used concentrated and diluted as in Experiment II. Result: Dilutions 
1, f, ^ all prevented clotting completely. 
Experiment IV. The glands of 10 A. persicus (ff, 5'5 to 7'2 mm. 
long) were emulsified separately in '02 c.c. of salt solution and each 
mixed with equal volumes of blood : 
The glands of 
Tick No. 
Delayed 
coagulation for 
Size of Tick 
(length) in mm. 
1 
2 
a 
4 
5 
6 
7 
8 
9 
10 
90 minutes 
45 „ 
45 „ 
prevented completely 
45 minutes 
75 
75 
75 
95 
75 
>> 
J > 
>> 
>> 
» » 
7'2 
7 
6 - 5 
6-2 
6 
6 
6 
6 
5-7 
55 
Experiment V. This experiment was made to determine if the 
emulsion of tick salivary gland exerted any deleterious effect on living 
leucocytes maintained at 35° C. The emulsion was mixed with a 
minute drop of human blood and the leucocytes were observed under 
the microscope. 
(a) One gland was emulsified and mixed with blood. 
Effect : Nil. Leucocytes actively motile after 2 hours. 
( b) One gland in strong emulsion was mixed with blood. 
Effect: Nil. 
In none of the foregoing experiments was there any haemolysis 
observed even when gland emulsion was added in large amount to the 
blood. 
