308 
Anticoagulin in Ticks 
A. persicus was, therefore, prepared and injected subcutaneously into a 
mouse. The injection produced no noticeable effect. Salivary gland 
emulsion was similarly injected into a mouse (glands of 6 Argas), a cock 
(glands of 5 Argas ) and a rabbit (glands of 5 Argas), but in no instance 
was the slightest local or general effect produced. 
The effect of temperature upon the anticoagulin in the 
salivary glands of A. persicus. 
In the case of the specific anticoagulin for rabbit’s blood 1 Bordet 
and Gengou (1901) found that the antibody resists heating to 58’5° C. 
Sabbatani states that the anticoagulin present in Ixodes ricinus is 
destroyed by an exposure to 100° C. for 5—10 minutes. 
To determine the temperature at which the anticoagulin in A. per¬ 
sicus is rendered inactive, we proceeded as follows: The glands of 
several ticks were dissected out and emulsified in salt solution, after 
which the emulsion was drawn up into capillary tubes. Some of the 
tubes were set aside as controls, others were heated to various tempera¬ 
tures for a period of 10 minutes. The capillaries which were heated 
were attached by elastic bands to a thermometer which was kept 
moving to and fro in a waterbath maintained at the temperature desired. 
After having been heated the emulsion was mixed with blood and 
tested in the usual way. 
In a preliminary experiment, made with the glands of one tick, the 
unheated emulsion prevented coagulation for over 4f hours, whereas an 
emulsion heated to 55° C. was to some extent inactivated since complete 
coagulation of the blood with which it was mixed took place after 90 
minutes. 
In the next experiment an emulsion of the glands of several ticks 
was used. In the tube containing unheated emulsion coagulation 
commenced to take place after G1 minutes, whereas the heated tubes 
gave the following results:— 
Heated to 
65° 
70° 
75° 
80° 
Complete coagulation after 
23 minutes 
16 
12 
f) 
>) 
8 
)> 
1 Produced by injecting rabbit’s blood into guinea-pigs. See Nuttall (1904), Blond 
Immunity and Blood Relationship, p. 17. 
