311 
INOCULATION OF DOGS WITH THE PARASITE OF KALA 
AZAR (HERPETOMONAS [LEISHMANIA] D0N0VAN1) 
WITH SOME REMARKS ON THE GENUS HERPETO¬ 
MONAS. 
By Captain W. S. PATTON, I.M.S. 
Since the discovery of the parasite of Kala Azar by Lt.-Col. 
Leishman, R.A.M.C., a number of investigators have endeavoured to 
reproduce the disease in the lower animals but without success. The 
recent discovery of Nicolle that dogs are susceptible to an allied human 
parasite ( Herpetomonas infantum ) has added a fresh stimulus to work 
along these lines, and in order to see whether the dog can be infected 
with H. donovani I carried out some inoculation experiments, which I 
propose recording in this paper. 
Three young dogs were selected from a number of specimens brought 
to the King Institute; they had no ticks on them nor were they 
infected with Piroplasma canis and beyond being somewhat emaciated 
they were good specimens of bazaar dogs. Through the kindness of 
Lt.-Col. Robertson, I.M.S., I was able to obtain lOc.c. of splenic blood 
from two typical cases of Kala Azar, and on examining some films it was 
found to be rich in parasites. 
The dogs were inoculated as follows : 
Dog A. 
Date 
9 June, 1908. 
Weight 
550 grms. 
Inoculated with 2 c.c. intra-hepatic and 
2 c.c. intra-peritoneal. 
Dog B. 
9 June, 1908. 
306 grms. 
Inoculated with 2 c.c. intra-hepatic and 
2 c.c. intra-peritoneal. 
Dog C. 
9 June, 1908. 
1124 grms. 
Inoculated with 2 c.c. intra-peritoneal. 
The inoculations were carried out with the greatest care, so that 
there could be no doubt that all the dogs received large numbers of the 
living parasites. The dogs were kept in clean iron cages free from 
ticks, their blood being examined at intervals with negative results. 
On June 19th the dogs were weighed and found to be as follows: 
Dog A, 820 grams, Dog B, 392 grams and Dog G, 1246 grams. On 
Parasitology i 20 
