G. H. F. Nuttall and G. S. Graham-Smith 225 
VI. Dog “ 9 ” (2 years old) treated with atoxyl before and after inoculation. 
The treatment before inoculation lasted three weeks during which period the dog 
received 1 °/ 0 atoxyl solution in doses increasing from 2 to 12 c.c. After inoculation 
it received 2% solution, the total amount given being equal to 53 c.c. of a 
2 °/ 0 solution. 
Day 1. Inoculated with 3'5 c.c. of piroplasma blood. 14 c.c. of a 1% atoxyl 
solution was given on this and the day following. 
4. Received 4 c.c. of 2 % atoxyl solution. 
5. Received 4 c.c. of 2 °/ 0 atoxyl solution. Parasites first observed in the 
blood. 
6. Received 4 c.c. of 2 °/ 0 atoxyl solution. Parasites numerous. Haemo- 
globinuria. 
7. Received 4'5 c.c. of 2 °/ 0 atoxyl solution. 
8. Fewer parasites. 
9. „ „ Received 4 - 5 c.c. of atoxyl 2 °/ 0 solution. 
10. Dog very weak. Received 4 c.c. of atoxyl 2 °/ 0 solution. 
13. Dog too weak to stand. Received 3 c.c. of atoxyl 2 °/ 0 solution. 
14 and 15. Few parasites found. 
16. Dog died. Death due to collapse and piroplasmosis. 
Control to experiments V and VI : old dog inoculated with 4 c.c. of piroplasma 
blood. Parasites first found on the 4th day. This dog was afterwards treated with 
atoxyl every two days, the dose being raised from 4 to 8 c.c. (In all an amount 
equal to 70 c.c. of a 2 °/ 0 solution of atoxyl was given.) 
Day 5. Parasites first found. Haemoglobinuria. 
10. Dog appeared better. Haemoglobinuria. 
12. Haemoglobinuria continues. 
18. „ „ Very few parasites. 
22. No parasites found in the blood. 
23. Dog appears well. 
25. Dog killed. Autopsy: spleen large, kidneys inflamed, and many 
parasites found in kidney smears. 
Result. Atoxyl treated dog (V) was killed on the 9th day, and was found to be 
suffering from a heavy infection. Dog VI died from piroplasmosis on the 16th day. 
The control dog had apparently almost recovered on the 25th day. (Although 
considered as a control by Gonder this dog received more atoxyl than any of the 
other animals.) 
Gonder’s experiments show that atoxyl does not favourably influence 
piroplasmosis in dogs. It does not retard the appearance of the parasites 
or delay the progress of the disease. In fact the symptoms appear to 
be aggravated. The drug by itself produces similar effects to piroplas¬ 
mosis, haemoglobinuria, bloody stools, great inflammation of the kidneys, 
and death due to cardiac failure (see Uhlenhuth, Htibener and Worthe, 
1908, p. 277), so that satisfactory results could scarcely be expected. 
