G. H. F. Nuttall and Gr. S. Graham-Smith 249 
at 26° C. After 20 hours a few forms such as are depicted in Plate XIX, 
figs. 2—4, were seen in corpuscles which had lost their haemoglobin, 
but none with true radii were found. 
Dog III. Several cultures were prepared in 0 - 8°/o salt solution and 
examined at various times, but no forms of interest were met with. 
Dog IV. Cultures were made in 0• 6°/ 0 and 0'8°/ 0 salt solution and 
kept at 24° C. for 27 hours. Several preparations made from the surfaces 
of the corpuscular layers of various cultures all showed numerous 
parasites with radiating processes from which the specimens shown in 
Plate XIX were drawn. 
Preparations made from the other layers and the supernatant fluid, 
showed very few if any parasites with radii. 
Dog V. Ten cultures were made in 0'6°/ 0 salt solution and kept at 
24° C. Examinations at 17 and 25 hours were all negative as regards 
parasites with radiating processes. After 41 hours a single form with 
true radii was found. 
Dog VI. A very complete series of cultures and examinations were 
made in this case. Cultures in 0’6°/ 0 salt solution were kept at 24° C. 
Preparations made after 15 hours and after 25 hours showed no 
parasites with radii. Examinations after 43 hours, however, showed 
numerous large forms and some with true radii. These forms were 
still present a few hours later. 
No forms of interest were noted in cultures made with 0'8 °/„ salt 
solution after 25 hours. After 43 hours several large forms were seen 
and one with true radii. Five hours later these forms were more 
numerous. 
In “P” solution no interesting forms were seen after 15 and 
24 hours. After 43 hours three forms with radiating processes were 
noted, and a few after 48 hours. 
Dog VII. In this case all cultures were kept at 24° C. and were 
left absolutely undisturbed until they were examined. 
In 0'6 % salt solution after 28 hours parasites were numerous and 
a few forms with radii were seen. After 27 hours’cultivation no obvious 
changes were noticed, but after 73 hours many forms with well marked 
radiating processes were seen. 
In 0’8°/o salt solution a few forms with radii were seen after 
28 hours, and after 47 hours they were numerous, but after 73 hours 
their numbers had decreased. 
In “ P ” solution no forms with radii were seen after 28, 47 and 
73 hours’ cultivation. 
