Gr. H. F. Nuttall and Gr. S. Graham-Smith 251 
at rest gave better results than those which were occasionally or con¬ 
tinuously shaken, or through which a current of air was intermittently 
passed. The times at which the forms with radiating processes were 
most numerous varied in the successful cultures. In dog VIII they 
were numerous after 17 hours’ cultivation, and were fewer after 
44 hours. In most cases however they were most numerous on the 
second day. After that time they usually decreased in numbers. 
Positive experiments. 
Temperature 
Dog 
Age 
At death 
_A_ 
Mode of rise 
Condition wheii 
blood taken 
Date after 
inoculation 
Days after 
fever 
commenced 
I. 
puppy 
103 °F. 
— 
stationary 
3 days 
2 days 
IV. 
9 9 
103-8 
slow 
falling 
9 „ 
4 „ 
V. 
99 
105-8 
9 9 
stationary 
7 „ 
5 „ 
VI. 
99 
106 
99 
99 
6 „ 
2 „ 
VII. 
9 9 
104 
99 
9 9 
4 „ 
0 „ 
VIII. 
99 
103-8 
9 9 
9 9 
6 „ 
*9 
a 9 9 
IX. 
9 9 
103-8 
rapid 
falling 
9 „ 
1 „ 
XIV. 
9 9 
103 
slow 
9 9 
6 ,, 
2 „ 
Negative 
experiments. 
n. 
puppy 
101-4° F. 
slow 
falling 
12 days 
6 days 
hi. 
99 
103 
rapid 
stationary 
10 „ 
2 
9 9 
X. 
99 
— 
? 
99 
5 „ 
1 „ 
XI. 
2 years 
104 
rapid 
9 9 
4 „ 
2 
" 99 
XII. 
old 
103-8 
99 
99 
5 „ 
1 „ 
XIII. 
puppy 
103-6 
99 
9 9 
5 „ 
1 „ 
XV. 
99 
103-6 
99 
99 
6 ,, 
1 „ 
XVI. 
99 
102-2 
very little ,, 
7 „ 
3 ,, 
XVII. 
99 
104 
rapid 
9 9 
4 „ 
1 „ 
XVIII. 
9 9 
103-6 
slow 
9 9 
5 „ 
2 
w 9 9 
XIX. 
99 
— 
— 
— 
— 
— 
Description of the cultivation forms. 
In cultures made in 0 - 6 °/o an( I 0'8 °/ 0 salt solution forms of the 
parasite may often be seen, which apparently are not degeneration 
forms, and which are never met with in the circulating blood or in the 
organs. The majority of these are large intracorpuscular forms. In 
very many cases the infected corpuscle has lost its haemoglobin, and its 
contour can only be made out with difficulty by following the faint line 
marking the rim of the collapsed and often much enlarged corpuscle. 
Occasionally no corpuscular remains can be defined and the large 
parasite appears to be free. Nevertheless we are inclined to think that 
16—5 
