252 Cultivation of Piroplasma 
most of these apparently free forms are really intracorpuscular, and 
that failure to find the corpuscular envelope is due to its extreme 
delicacy and lack of staining power. 
The least differentiated forms are large definitely intracorpuscular 
parasites, such as are figured in Plate XIX, figs. 2—4. Some of these 
show two well defined masses of chromatin (fig. 1) while others, usually 
of irregular shape, show three or more masses (figs. 2, 3, 4). Very 
rarely extremely large forms are seen with several masses of chromatin 
apparently connected together by thin strands of chromatin (fig. 21). 
Other examples of intracorpuscular parasites of the same general type 
show minute, delicate, radiating processes (previously described as radii) 
projecting beyond the parasites, and usually originating in the neigh¬ 
bourhood of a chromatin mass (figs. 5, 6). Others show much longer 
processes, frequently long enough to reach the margin of the corpuscle 
(figs. 7, 8, 9). These processes, which are often extremely delicate, 
especially at their distal extremities, vary greatly in number, sometimes 
being almost too numerous to count (fig. 12). 
Occasionally several large parasities of this type are seen in one 
corpuscle (fig. 20). 
In yet more remai'kable forms some of the processes cause small 
projections or even considerable distortions of the corpuscular envelope 
(figs. 9, 11, 13), and occasionally may even perforate the envelope and 
project for a considerable distance beyond it (figs. 13—16). Similar 
forms (figs, 17, 18, 19), apparently free, are occasionally seen. 
In living preparations all intracorpuscular parasites, except some of 
the rounded forms, show slight movements, probably of a molecular 
nature, since small masses of detritus with active dancing movements 
are often seen within the envelopes of the collapsed corpuscles. 
On several occasions we have kept under observation living examples 
of the forms with long processes and have noticed changes of shape 
accompanied by the very slow protrusion and retraction of the radiating 
processes. The accompanying figure illustrates the changes noticed in 
one parasite (A) during 50 minutes’ observation, and in another (B) 
during a period of five minutes. 
Owing to the uncertainty of the cultural methods and the difficulty 
of keeping living parasites under observation for long periods we have 
been unable to follow the development of these forms. Possibly the 
forms with numerous processes develop from forms with many chromatin 
masses (fig. 21) and the latter by fusion from multiple intracorpuscular 
forms such as are illustrated in fig. 20. 
