G. H. F. Nuttall and Gr. S. Graham-Smith 253 
In the absence of observations on living parasites the interpretation 
of the appearances seen in stained preparations is extremely hazardous 1 
and we do not feel justified in offering any conjectures as to the origin 
and significance of the bodies we have described. 
Appendix. 
1. Attempts to cultivate the other species of Piroplasma. 
Lignieres (cited by Chauvelot, 1904, p. 11) using blood containing 
many parasites did not succeed in cultivating P. bovis in different kinds 
of broth, gelatin, agar, or on potato, serum, amniotic fluid, or aqueous 
humor, whether the cultures were exposed to the air, kept in an 
atmosphere of carbon dioxide, or hydrogen, or kept in vacuo. He also 
obtained negative results with blood kept in collodion sacs. As a 
culture medium he also tried serum rich in haemoglobin obtained from 
diseased cattle, adding to it 0'25 c.c. of blood containing many parasites, 
1 Breinl and Hindle (1908) have recently asserted that P. canis multiplies in the blood 
in several different ways. Observations on the forms assumed by the parasite in the 
living blood during multiplication, which can be studied without great difficulty, do not 
lend any support to their hypotheses, which are based on the evidence derived from wet 
films stained by Breinl’s method. 
