180 
Protozoa in Tipula 
sometimes placed excentrically, and also a few chromatin grains in the 
surrounding nuclear sap. There is generally a distinct group of extra- 
nuclear siderophilous granules. 
The flagella can be seen to take their origin in two basal granules, 
which are sometimes connected by a dark-staining delicate line (Fig. 11). 
An axostyle is usually developed, and appears to arise from one of the 
basal granules. It then curves around one side of the nucleus, and 
runs as a slightly sinuous line to the posterior end of the body, beyond 
which it seldom projects. (Cf. the more rigid axostyle of trichomonads.) 
The vacuolated cytoplasm contains numerous inclusions, sometimes of 
astonishing size, considering that there is no hint of a cytostome. The 
flagellate is sometimes parasitized by a micrococcus. 
Fig. 10. Monocercomonas from Tipula. Flagellate individual. 
Fig. 11. Monocercomonas. Flagellate showing connection between basal granules. 
The Monocercomonas from Tipula agrees in all essentials with the 
closely related species M. melolonthae (Grassi) (1879) from Melolontha 
and Gryllotalpa, and M. cetoniae Jollos (1911)h 
1 M. bufonis Dobell (1908), the only other well-authenticated species in this genus, 
differs markedly from these in having no axostyle, and in possessing a well-developed 
“ pseudo-chromidial body.” (Alexeieff, 1911.) 
