272 Herpetomonacls 
6. In encystment the tiagellum is not cast off bodily, but is drawn 
down into the cell by the kineto-nucleus, which moves to a position 
either alongside of, or posterior to the tropho-nucleus. In this way 
apparent Crithidia or even trypanosome forms are produced, but there 
is no hint of an undulating membrane. Small blunt “trypanosomes” 
were also produced occasionally by adhesion of the flagellum to the body- 
wall in the “stickiness” resulting from confinement under a cover-glass: 
the efforts of the flagellum to free itself raised up an undulating 
membrane, and produced a very deceptive appearance. 
7. The early stages of encystment could be induced by keeping the 
flagellates under waxed-down cover-slips, where they would continue to 
live for 30—40 hours. Similar results were got by transferring flagel¬ 
lates to agar-agar, or by subjecting the larval host to starvation for a 
day or two. 
8. From what I have seen, I do not agree with Chatton and 
Alilaire’s suggestion to divide the genus Herpetomonas into Leptomonas 
and Herpetomonas proper. I do not think that these authors are 
justified in regarding the double flagellum and the presence of a long 
rhizoplast as generic characters. 
REFERENCES. 
This list contains the latest additions to the literature on the herpetoinonads. 
Fuller references may be found at the ends of some of these articles. 
Aders, W. M. (1909). Herpetomonas aspongopi. Parasitology , ii. No. 3, pp. 202-7. 
2 text-figs. 
Alexieff, A. (1909). Les flagelles parasites de l’intestin des batrachiens indigenes. 
Compt. rend. Soc. Biol., lxvi. pp. 199-201. 
Berliner, E. (1909). Flagellaten-Studien. Arch. f. Protistenk., xv. pp. 297-325. 
2 pis. 
Chatton, E. (1909). Sur un trypanosomide nouveau, Leptomonas agilis, d’une 
reduve indigene ( Harpactor iracundus, Scop.). Compt. rend. Soc. Biol., lxvi. 
pp. 981-2. 
- (1909). Sur un trypanosomide nouveau d’une Nycteribie, et sur les relations 
des formes Trypanosoma, Herpetomonas, Leptomonas et Crithidia. Ibid., LXVII. 
pp. 42-4. 5 text-figs. 
Chatton, E. and Alilaire, E. (1908). Co-existence d’un Leptomonas ( Herpetomonas) 
et d’un Trypanosoma chez un muscide non vulnerant, Drosophila confusa, 
Staeger. Ibid., lxiv. p. 1004. 1 text-fig. 
Hinble, E. (1909). The life-history of Trypanosoma dimorphon. Unit). California 
Publications. Zool., vi. pp. 127-44. 3 pis., 1 text-fig. 
Leger, L. (1902). Sur la structure et la mode de multiplication des flagelles du 
genre Herpetomonas, Kent. Compt. rend. Acad. Sc., cxxxiv. pp. 781-4. 7 figs. 
- (1903). Sur quelques Cercomonadines nouvelles ou peu connues parasites des 
insectes (note preliminaire). Arch. f. Protistenk., ii. pp. 180-9. 4 figs. 
