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large ovoid and bacillary forms, some budding, some showing irregular 
swellings along their course, others again being club-shaped (see Plate 
XXI, fig. 3). 
On other solid media, such as ordinary serum, they grow in much 
the same way as on agar. They do not change the colour of neutral- 
red agar, and form a light red shining film on the top of the stab 
culture. On potato at 22° C. the organisms form within two to thi-ee 
days a moist shining yellowish film, which spreads slowly. Potato 
cultures at 37° C. scarcely show any growth. 
The growth of this organism in gelatin stab cultures is most 
characteristic. Two or three days after inoculation the organisms grow 
in a fine line along the track of the needle, and form a delicate bluish 
or yellowish white film on the top. After from one to two weeks, a 
short radiating outgrowth appears along the track of the needle, 
whereas the growth on the top represents a sort of “corona radiata,” 
with a central shallow pit. The organisms continue to grow slowly in 
this regularly radiating manner on the top, and, after 3 or 4 weeks, the 
single “ radii,” starting from the periphery, split up into separate 
segments or leaflets, so that the appearance of a “ Daisy-Head ” results 
(see Plate XXI, fig. 5). The separate leaflets soon cease to grow with 
the same regularity as hitherto and in cultures two to four months 
old, the growth on the surface of the gelatin is somewhat fernlike. 
In broth, the D. M. develops still more peculiar and irregular forms 
than on acid media, the most remarkable feature being the development 
of long filaments and threads ; besides there are large spheroid and 
ovoid forms, with or without budding, and chains of large round bodies. 
Sometimes a sort of false branching of the filaments can be seen, but I 
also observed instances of true branching (see Plate XXI, fig. 4). The 
broth shows in 24—28 hours after inoculation a general turbidity, but 
later on clears up, a deposit being formed at the bottom. If the 
organisms be again subcultured on ordinary solid media (agar, serum, 
etc.) the coccoid forms reappear (as iD Plate XXI, fig. 2). 
The same growth as in broth occurs in other liquid media, peptone 
water, milk, etc. 
Milk remains unchanged. Indol is not formed. 
The organisms grow aerobically and anaerobically, either at 22° C. 
or 37°C. 
