N. H. SWELLENGREBEL AND 0. STRICKLAND 
365 
some authors as a reduction of the nucleus and blepharoplast are 
probably nothing but such degenerating forms; so we will not de¬ 
scribe them here, but merely give some characteristic types showing 
fragmentation of the nucleus (Diagram I, Figs. 3, 6), Irypertrophy 
and fragmentation of the blepharoplast (Figs. 4, 5, 7). Other forms 
are identical to those found in the blood of the rat, with an 
alveolar protoplasm and a nucleus with one or two central chromatic 
granules (Fig. 1). The “never dried” preparations stained after 
Heidenhain’s method exhibit absolutely the same structure (Fig. 2). 
The trypanosomes become very soon agglomerated when the gut 
wall is ruptured; this phenomenon might be falsely interpreted as 
conjugation, because the flagellates often agglomerate in pairs. 
Twenty-four hours. The greater part of the trypanosomes in the 
midgut exhibit the following changes in their structure. The ble¬ 
pharoplast leaves its position at the posterior end of the flagellate and 
wanders in the direction of the nucleus (Diagram II, Fig. 1). The 
Figs. 1—5. Different stages of the wandering of the nucleus and the blepharoplast. G. 
nucleus on the other hand moves toward the hind-end of the cell 
(Fig. 2). So the distance between the nucleus and blepharoplast 
diminishes gradually (Fig. 3). Finally, the two pass each other 
(Fig. 4), and the blepharoplast comes to lie anterior to the nucleus, 
the trypanosome having now a Grithidia facies (Fig. 5). 
These changes bear a strong resemblance to those described by 
Chagas (1909) in Sckizotrypanum cruzi in the gut of Conorrhinus, but 
we never saw any sign of fusion of the nucleus and the blepharoplast. 
The structure of the nucleus is the same as in the ordinary trypano¬ 
somes, the blepharoplast often appears to be composed of distinctly 
