J. A. GrILRUTH, G. SWBET AND S. DoDD 
3 
were sufficient to indicate that these bodies could apparently be trans¬ 
ferred to other animals of the same species by inoculation and by skin 
parasites, and further: “media of all kinds inoculated with blood 
remained sterile, while blood within sterile pipettes placed at blood 
heat did not alter, and showed no increase, but rather a decrease, of the 
bodies described.” 
They were also in February of this year (1910) found by one of us 
(J. A. G.) in two Victorian pigs which were in an unthrifty condition due 
to dietetic errors as indicated by their rapid recovery on being supplied 
with proper food. During the period of observation, seven to ten days, 
there was no manifest change in the number of anaplasms. They have 
since also been found in healthy pigs. 
The special interest of this note however lies in the discovery of these 
bodies in the blood of monotremes and marsupials. 
General Description. 
The bodies found by us in the animals noted above correspond to 
Theiler’s description of Anaplasma marginale. With Giemsa’s and 
similar stains they stain a deep blue with clear cut outline, and vary in 
diameter from 0'9 p to 2'6 p. Both intra- and extra-corpuscular bodies are 
frequent. When within the erythrocyte they may occupy any position, 
although the great majority are to be found near and sometimes at the 
margin. The non-staining polar zone is not infrequently found. Double 
forms, probably fission products, are also to be seen, usually free, but also 
intra-corpuscular. A smear of blood from one Pseudochirus peregrinus 
shows, in a field of 0‘17 mm. containing 27 anaplasms, seven double 
forms. The number present varies considerably in the different animals 
but they are quite as numerous in smears from apparently healthy 
living marsupials and monotremes as in those from dead animals of 
the same species. In one specimen of Petaurus breviceps (immature), 
besides the usual forms, there were some others (apparently anaplasms) 
surrounded by a definite clear oval or round capsule (see Fig. 9). The 
body within this capsule varied in staining reaction, being generally in¬ 
distinguishable from the ordinary intensely stained anaplasm but at 
other times faintly stained (with Giemsa’s and Jenner’s stains) and more 
or less irregular or wedge-shaped. These encapsuled forms were found 
both intra-corpuscular and free, the oval forms being 3'5 p in length and 
3‘1 p in width, and the round forms 3’1 p to 3‘2 p in diameter. 
1—2 
