E. Kindle 27 
to assume, either the presence of lesions in the infected partner, or the 
occurrence of trypanosomes in the seminal fluid. 
The experiments recorded above show that in the case of T. gam- 
hiense there is no necessity for any lesion in the mucous membrane of 
the healthy partner for infection to take place, once the parasites are 
brought in contact with it. In restricting the spread of Sleeping 
Sickness it is important, therefore, to insist upon the necessity for 
personal pi’ophylaxis, even though infection other than by Glossina 
palpalis (and possibly G. morsitans) is probably exceptional. 
Note :—Since this paper was written Martin and Ringenbach^ have 
published an account of similar experiments with T. gamhiense in 
guinea-pigs. These authors obtained infection by introducing infected 
blood into the vagina of guinea-pigs and also by rubbing infected blood 
on the shaved skin. They failed to obtain infection, however, by 
spreading blood on the unshaven skin, except in the case of two females, 
in which the writers consider that the infected blood ran down the belly 
and entered the vagina of each of the animals. The experiments 
recorded above show that, in the case of rats, T. gamhiense may pass 
through the unshaven skin, for the experimental conditions were such 
that it was impossible for the infected blood to come in contact with 
any mucous membrane. 
1 Bull. Soc. Path. Exot. 1910, pp. 433—437. 
