44 
Piroplasma canis etc. 
6. Adults fed as nymphs on uninfected dogs and as adults on uninfected dogs. 
24. 8. 07. Adults fed on dog 21 (uninfected). 
3 dissected 24 days after dropping showed no cysts. 
27. 8. 07. Adults fed as nymphs on dog 11 (uninfected) and as adults on 
a negative dog. 
5 examined. None showed cysts. 
5. 9. 07. Adults fed as nymphs on dog 12 (uninfected) and as adults on 
dog 33 (uninfected). 
4 dissected on dropping showed neither vermicules nor cysts. 
9. 9. 07. Adults fed on dog 38 (uninfected). 
1 dissected showed no vermicules or cysts. 
11. 9. 07. Adults fed as nymphs on dog 12 (uninfected) and as adults on 
dog 21 (uninfected). 
8 examined for vermicules. All negative. 
3 examined for cysts. All negative. 
7. Adults fed as nymphs on uninfected dogs and as adults on infected dogs. . 
24. 8. 07. Adults fed on dog 23 (haemogregarines). 
4 examined for vermicules and cysts showed numerous vermicules 
but no cysts. 
5. 9. 07. Adults fed on dog 29 (fair number of haemogregarines) and allowed 
to commence oviposition. 
3 examined. Oocysts present in all. No large cysts present. 
24. 8. 07. Adults fed on dog 11 (numerous haemogregarines) examined 
24 days after dropping. 
Every specimen examined showed half-grown or nearly mature 
cysts. In many cases these were stained yellow and appeared 
as though no longer developing. 
Numerous unrecorded experiments also showed that ticks fed on 
infected dogs showed vermicules or some of the various stages 
(depending upon the time when examined) described in my 
first paper or referred to in the first portion of the present one. 
8. Adults fed as nymphs on infected dogs but as adults on uninfected dogs. 
28. 8. 07. Adults fed as nymphs on dog 9 and as adults on dog 21 (uninfected). 
6 dissected. All showed cysts but none vermicules. 
1. 9. 07. Adults (source unknown but with free access during nymphal stage 
to numerous infected dogs) fed on dog 21 (uninfected). 
3 dissected. Cysts were present in two. None showed vermicules. 
2. 9. 07. 2 more adults from laboratory fed on dog 21. One showed cysts; 
neither showed vermicules. 
These experiments, tliough more limited in number than was 
originally intended, are sufficient to prove that the developmental cycle 
dealt with was that of the haernogresfarine. 
O O 
The Development of Piroplasma in the Tick. 
During the experiments with Leucocytozoon canis a number of ticks 
were examined which had been fed on dogs infected with Piroplasma 
canis. In such cases developmental forms as described by me in a 
previous publication were frequently seen. 
