S. R. Christophers 
45 
Tlie club-shaped forms are the most easily detected stage in the 
developmental cycle and can generally be found without difficulty about 
the fourth or fifth day in ticks which have been fed on heavily infected 
dogs. For their detection fresh preparations are made of the ovary, 
oviducts etc. and the field examined under a comparatively low power 
for the actively motile small leech-like immature club-shaped bodies or 
the more rigid and less active maturer forms. 
Sporozoits in 
Diagram 2. Showing the developmental changes of 
Piroplasma cants in the tick. 
Since ray original description was published several authors have 
confirmed the view that the various appearances described by Prof. Koch 
and myself are stages in the development of Piroplasma. Marzinowski 
and Bielitzer’s (s) work is the most detailed. They describe the develop¬ 
ment for P. equi as follows : 
“ One finds the star-shaped forms of Koch on the first, less frequently 
on the second day. They are, however, seldom to be found in stained 
preparations. Therefore it would be unwise to give them any great 
