358 
Regeneration in Argas persicus 
without another feed in order to see whether the absence of a limb 
would stimulate the animals to proceed to another moult, but they all 
died without proceeding to another stage. 
Experiment 5. In all the preceding experiments only the second 
right leg had been removed and therefore we sought to determine if the 
removal of other limbs gave corresponding results. The larvae used in 
this series were all put on a fowl on April 5 and dropped off gorged five 
days later. After amputation some of these larvae were kept at 37° C. 
and others at 30° C. For the sake of convenience the results are 
presented in tabular form. 
(a) Larvae kept at 30° C. 
i. First right leg amputated 10. iv. 13 : 
16. iv. 13 9 moulted No regeneration. 
17. iv. 13 3 
ii. Second right leg amputated 10. iv. 13 : 
17. iv. 13 8 moulted No regeneration. 
18. iv. 13 4 ,, ,, 
iii. Third right leg amputated, 10. iv. 13: 
16. iv. 13 5 moulted No regeneration. 
17. iv. 13 6 
iv. Hind pair of legs amputated 10. iv. 13: 
16. iv. 13 2 moulted No regeneration. 
17. iv. 13 6 ,, „ 
18. iv. 13 3 ,, In two examples, no regeneration ; in the third, minia¬ 
ture legs one-third the normal size were present. 
v. Two hind pairs of legs amputated 10. iv. 13 : 
16. iv. 13 7 moulted No regeneration. 
17. iv. 13 5 ,, ,, 
vi. All three pairs of legs amputated 10. iv. 13: 
17. iv. 13 3 moulted No regeneration. 
18. iv. 13 7 ,, No regeneration in eight individuals ; in two cases a two - 
jointed knob developed in place of the front legs. 
With few exceptions the ticks in this series all moulted without 
showing any signs of regeneration, and it will be noticed that the period 
elapsing before moulting is only slightly lengthened in the case of those 
animals which had lost all their limbs. In all the above series the 
fourth pairs of nymphal legs appeared as usual and were normal both in 
size and form. 
( b ) Larvae kept at 37° C. 
i. First right leg amputated 10. iv. 13 : 
14. iv. 13 4 moulted 3, no regeneration ; 1, small two-jointed knob regenerated. 
15. iv. 13 1 ,, Small three-jointed rod developed, with miniature claw. 
