E. Hindle and N. Cunliffe 
367 
Experiment 12. As in the case of the first stage nymph, we performed 
a series of experiments in order to determine whether the regenerative 
power varied in different appendages, and also after the removal of 
different articles. 
A batch of second stage nymphs was fed on May 5 and the gorged 
ticks amputated in various ways on the following day. 
(a) Tarsus of second right leg amputated 6. V. 13. 
20. v. 13 
21. v. 13 
22. V. 13 
24. v. 13 
2 moulted 2 © 
1 ,, O 
2 ,, 3 + 0 
1 ¥ 
As a rule the three distal articles of the 
regenerated limb distinctly shortened and 
whole leg slightly reduced. 
( b ) Two distal articles of second right leg amputated 6. v. 13. 
21. v. 13 3 moulted 3 + ¥ + G i In all cases the regenerated leg was approxi- 
22. v. 13 2 ,, 3 + 3 - mately two-thirds normal size; all articles 
23. v. 13 1 ,, ¥ J reduced in size. 
(c) Three distal articles of second right leg amputated 6. v. 13. 
20. v. 13 1 moulted © 
22. v. 13 1 © 
23. v. 13 1 3 
24. v. 13 3 ,, 3 ¥ ¥ 
The regeneration was of the same type as 
that in (&). 
Fig. 4. Argas persicus ; (A) third stage nymph from Experiment 12 (e), showing regenera¬ 
tion after removal of all the appendages ; ( B) normal tick for comparison. ( x 13.) 
24—2 
