No. 2.] COMPARATIVE CYTOLOGICAL STUDIES . 307 
kleinen, gefarbten Kiigelchen besteht.” In the egg of Anabolia 
there is one large nucleolus, but in those of Vespa germanica 
and V. media apparently no true nucleoli are present. There 
is a large granular nucleolus in the larger germinal vesicles 
of Bombas terristris. Trogas lutorius: there is one large, 
irregularly shaped nucleolus and two smaller ones; all these 
finally disappear, and their place is taken by smaller granules. 
Banchus falvipes: at first no nucleolus is present, later one or 
three large nucleoli appear, but all of them vanish subsequently. 
In the egg nucleus of Pimpla sp. only a number of small 
granules are to be found, and at a later period still smaller 
granules. Anomalon circumflexum: in the youngest germinal 
vesicles no nucleolus is to be found, in older ones there is 
a single large one; this has nothing to do in the formation of 
the “ Dotterkerne,” and disappears when the nucleus does. 
There is one spherical germinal spot in Ophion ventricosum } 
but not in O. lateum. Ephialtes liturater: in the smaller nuclei 
a considerable number of “ chromatic ” bodies occur, while in 
the older ones there is a single large nucleolus. Ambyteles 
castigator: one large nucleolus, in older ova also several smaller 
ones. Epeira diademata: here is one large spherical nucleolus, 
which later becomes jagged in outline and evinces vacuoles, 
which may unite to produce a single larger vacuole : “ In sel- 
tenen Fallen kann man einen Zerfall des Nucleolus in mehrere 
kleinere sehen, was jedoch wohl eine pathologische Erscheinung 
sein diirfte.” Glomeris marginata: one large, spherical or 
angular nucleolus, and later also a smaller one : “ Hochst 
wahrscheinlich stammt dieser von dem grossen Nucleolus ab ” ; 
the smaller nucleolus disappears subsequently. In the egg of 
Peripatus edwardsii one nucleolus forms itself gradually, and 
vacuoles begin to appear in it. In Amaroecium rubicundum a 
single large nucleolus is present; while in Clavelina lepadi- 
formis the nucleolus is probably formed out of the central 
chromatin masses. From these numerous observations Stuhl- 
mann draws the conclusion: “ Aus Allem schien mir hervorzu- 
gehen, dass das Schwinden des Nucleolus nicht zum Wesen 
der Eireifung gehort, besonders weil ich ihn bisweilen (so bei 
Silpha) so lange verfolgen konnte, als noch ein Rest des 
Keimblaschens im Ei sichtbar war.” 
