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or so) of globules of varying size which appear to lie loose 
within the germinal vesicle. An intravesicular network is 
sometimes present, and serves to unite the granules of the 
macula. ... It is possible that the homogeneous matrix 
above described may represent the remains of such a network, 
the filaments of which have shrunk up into a mass on contact 
with the hardening reagent ” (picric acid and alcohol). 
Trinchese (’ 80 , according to Platner, ’86) found in the germinal 
vesicle of Amphorina coerulea a “macchia germinativa laterale 
o accessorial and a “macchia germinativa principale,” the 
latter being about seven times the size of the former. 
1881 . 
Balbiani (’ 81 ) investigated the salivary gland cells of the 
Chironomus larva. There are here “ Deux gros nucleoles irre- 
guliers, larges de 0.03 a 0.04 mm., bosseles a leur surface, et 
formes d’une substance refringente granuleuse, creusee d’un 
plus ou moins grand nombre de vacuoles isolees ou confluentes. 
II arrive assez souvent que les deux nucleoles se confondent par 
une partie plus etroite qui les reunit comme une sorte de pont; 
d’autres fois enfin, ils se fusionnent plus ou moins intimement 
en un seul nucleole, dont le diametre est le double de celui des 
nucldoles isoles.” The ends of the chromatin filament are 
apposed against the nucleolus; and the latter differs both 
chemically and morphologically from this “ cordon nucleaire.” 
Giard (’ 81 ) observed in the egg of a Spionid during life a 
single central nucleolus. A certain time before completed 
maturation an “Element cellulaire ” appears in the nucleus, 
which is a little smaller than the latter, and encloses in its 
center a small “ noyau ” : “ D’abord fort eloigne du nucleole, il 
s’en approche progressivement et vient s’appliquer a sa surface, 
ou il s’aplatit et prend la forme d’une double calotte. En 
s’appliquant de plus en plus contre le nucleole, il perd son 
noyau et finit par se reduire a une double membrane qui entoure 
le nucleole,” . . . and finally its substance fuses with that of 
the nucleolus. 
Hubrecht (’ 81 ), egg of Proneomenia : “ in all the different 
stages of development of the ovum the germinal spot is double: 
