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[Vol. XV. 
methylen green. In mitosis, when the nucleus is uninucleolar, 
the substance of this nucleolus becomes dissolved in the nucleus; 
when multinucleolar, however, one of the nucleoli may pass 
out into the cytoplasm. Hesperus, “ Pollenmutterzelle ”: here 
there is one nucleolus, which stains with methylen green, as does 
the chromatic filament, and disappears in mitosis. Pisium , 
same cells : here there is one hat-shaped nucleolus, which stands 
in no connection with the “ Fadenknauel”; “ Sehr eigenthiim- 
lich ist das Verhalten des Nucleolus in den die Kerntheilung 
vorbereitenden Stadien. Anfanglich besteht derselbe aus 
homogener, stark lichtbrechender Substanz. Spater sind am 
Nucleolus eine dichte aussere und eine innere, bedeutend 
schwacher lichtbrechende Schichte unterscheidbar. Endlich 
findet man Stadien, auf denen neben dem noch unveranderten 
Fadenknauel ein sehr schwach lichtbrechender Korper gefunden 
wird, dessen Umrisse vollkommen demjenigen des urspriing- 
lichen Nucleolus entsprechen ”; finally even this disappears. 
Zacharias (’ 82 ) studied the epidermis cells of Phajus , and 
concludes that the nucleoli (one or two in number) consist of 
plastin. They do not dissolve in distilled water; swell with 
.if) nitric acid ; do not stain with methylen green; and 
dissolve in weak “ Kalilaugelosung.” 
Heuser (’ 84 ) studied the mitoses in the embryo sac of Fritil- 
laria imperialis. In the resting nucleus there are from five 
to nine nucleoli: “ Dieselben sind intensiv gefarbt und stehen 
in deutlich wahrnehmbarea Zusammenhang mit dem Nucleo- 
Hyaloplasma.” In the prophase of the mitosis they lose their 
staining power and apply themselves to the chromatin threads. 
He considers them, with Strasburger, “als Reserve-Behalter 
der Kernsubstanz ” (using the term “ Kernsubstanz ” as equiva¬ 
lent to “Chromatin”); their ground substance consists of 
plastin, permeated with chromatin. In Fritillaria , as well as 
in Galanthus and Leucojum , “ fliesst das gesammte Kernkor- 
perchen in die Kernelemente iiber, wahrend in anderen Fallen 
ein Ueberschuss an Plastin als ‘ Secretkorper ’ ausgeschieden 
werden mag.” 
Strasburger (’ 84 ), nuclei in the embryo sac of Fritillaria: in 
the spirem stage the large nucleoli disappear, “ wobei sich um 
