406 
MONTGOMERY,\ 
[Vol. XV. 
akin to part of the substance of the nucleoli, I am unable 
to decide. I am also unable to determine from the prepara¬ 
tions at hand whether the nucleoli themselves are partially 
composed of chromatin ; but the usual diagnostic stains for 
chromatin do not show the presence of this substance within 
the nucleus. 1 
To revert again to the polarity of the nucleoli. The fact 
that the vacuoles first arise in that portion of the nucleolus 
nearest the nuclear membrane would seem to prove that the 
substance of these vacuoles is extranuclear in origin, or else is 
secreted in the peripheral portion of the nucleus. But since it 
would be obscure how the peripheral portion of the nucleus 
should secrete a substance, and the central portion should not, 
I incline to the former explanation, namely, that the substance 
of the vacuoles is first produced in the cytoplasm, and then this 
substance penetrating through the nuclear membrane, it, or a 
part of it, arrives at that pole of the nucleolus nearest the 
nuclear membrane, and then is taken into the nucleolus at 
this pole. The size of the vacuoles stands in a more or less 
direct ratio to the size of the nucleolus itself; at the same time 
the ground substance of the nucleolus also increases in amount, 
though apparently not as rapidly as the amount of the vacuolar 
fluid. 
2. Gregarine from Carinella annulata . 
(Plate 21, Figs. 20-35.) 
{Description of the animal .—Monocystid gregarines occurring 
in the body cavity of this nemertean. No synzigia observed. 
Form : elongate, though not attenuate, the end in which the 
nucleus lies being broader and terminally more obtuse than the 
opposite end (Figs. 20 and 21). The longitudinal axis is never 
perfectly straight, and the cuticula shows no transverse fur¬ 
rows. The single nucleus is usually spherical or oval, rarely 
lobular in outline. In the entosarc of many individuals occur 
numerous minute, refractive granules. Neither cysts nor spores 
having been observed, I was unable to determine the genus of 
1 However, the chromatin here might exist in the state in which it is found in 
the growth period of ovocytes, namely, commingled with plastin. 
