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MONTGOMERY. 
[Vol. XV. 
present, though variable in size and number. They do not 
regularly arise at one particular part of the nucleolus, as we 
found to be the case in the preceding species. Further, there 
is rarely in this species a single large excentric vacuole ; but 
as the figures show, usually a number are present, either 
arranged in a circular row near the periphery, or in a row 
around a larger central vacuole, or grouped together at one 
point in the nucleolus. There can be no doubt that the larger 
vacuoles are produced by the fusion of smaller ones, since two 
or three smaller ones are frequently found in close contact 
with each other. 
The double stain, haematoxylin and alum carmine, gives 
different results from the preceding stains, in that by it not 
only the different nucleoli within a nucleus become colored 
differently, but also in some cases different stains of the 
different portions of the same nucleolus are attained (Figs. 
22-25). It is only the larger nucleoli, those with regular con¬ 
tours, which become differentially stained in this manner. In 
such a large nucleolus a portion of its substance stains a deep 
blue (haematoxylin), another portion or portions purplish or 
reddish (alum carmine) ; the part stained blue is usually central 
in position, and encircling it is a zone of red-stained substance. 
In one case (Fig. 22) the two opposite poles of the nucleolus 
were reddish, the intermediate part being a deep blue. The 
medium-sized, irregular nucleoli always stain blue throughout, 
the smaller ones usually red, but sometimes blue. This stain, 
accordingly, shows that in this gregarine some of the larger 
nucleoli are composed of two different substances similarly as 
we had found two substances in the preceding species, though 
there by using the methylen-blue-brasilin stain. 
With all three staining methods employed, a mass of irregular 
granules is present in each nucleus, which stain less intensely 
than the nucleoli. In the smallest nuclei (Figs. 22-25) these 
granules are more or less regularly distributed through the 
nucleus, but in the larger ones (Figs. 28, 31-35) they com¬ 
pose a dense mass around the nucleoli or around the largest 
nucleolus, while the peripheral portion of the nucleus remains 
nearly free of them. Delicate, faintly stained fibers transverse 
