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and eosin (Figs. 68 and 69). With the Ehrlich-Biondi method 
this difference is not quite so clearly demonstrable. The latter 
stain is peculiar and differs from all other stains used by me 
for these cells, in that it very often gives to the smaller vacuoles 
of the nucleolus the appearance of black, refractive granules ; 
but a careful focusing of these supposed granules shows them 
without doubt to be vacuoles, their apparent solidarity being 
probably due to the refraction of light by the enveloping 
nucleolar substance. 
The chief result derived from the foregoing observations is 
that the nucleolus takes up some or all of those nutritive 
globules which lie in the caryolymph, and whose substance had 
been probably derived from the cytoplasm. Some of these glob¬ 
ules then become collected within the nucleolus, representing 
its fluid vacuoles ; and these globules, increasing in number at 
the same time, gradually fuse together and thus give rise to 
a single large excentric vacuole, which is enveloped by the 
unchanged true nucleolar substance. Since the substance of 
these small globules is probably nutritive in function, the 
nucleolus in thus collecting some or all of them would appear 
to act as a reservoir for nutritive substance, or as a reservoir 
for that portion of the nutritive substance accumulated in the 
nucleus, for which the nucleus may have no use. Of course 
it is not a priori impossible that these globules may represent 
waste products of a nutritive substance, so that the nucleolus 
might here fulfill the office of an excretory organ. But the 
function of these nucleoli can only be decided when the 
behavior of the nucleolus during the pole-body mitosis is 
known ; I had no ova showing pole-spindle formations. 
Finally, the true nucleolus appears not to be bounded by a 
special membrane ; after staining with acid carmine and nigro- 
sine the nuclear substance appears bluish green and a red 
membrane seems to envelop it (Fig. 80), but this appearance is 
probably due to the refraction of light, since nothing of the 
kind can be found after the use of other staining methods. 
We now come to speak of what I have called the “ pseudo- 
nucleoli/’ but merely in order to distinguish them from the 
true nucleolus, and without wishing to express by the use of 
