474 
MONTGOMERY. 
[Vol. XV. 
Colossal ganglion cells (Figs. 90-92, 94-97). —In the nuclei of 
these there are never more than from one to three nucleoli, 
which neither contain vacuoles nor become noticeably irregular 
in size, as is the case in Doto. Most frequently only a single 
nucleolus is present. It is the rule that they are oval and not 
spherical, though in some cases they may appear perfectly 
spherical ; perhaps the great majority of them are oval and 
seem to be spherical only when they do not chance to be longi¬ 
tudinally sectioned. Their substance is perfectly homogeneous, 
without a limiting membrane. When two or three occur in the 
same nucleus they are usually of approximately equal dimen¬ 
sions (Figs. 94 and 95). Further, it would seem to be the rule 
that when one nucleolus is present in a nucleus it is larger than 
any one of the two or three which may be found in other 
nuclei ; but, nevertheless, the relative amount of nucleolar 
substance seems to vary in different nuclei. 
Ganglion cells of medium size (Fig. 93). — Here one or two 
nucleoli are present in each nucleus, and these are of homo¬ 
geneous appearance. 
Smallest ganglion cells. — The nucleoli are similar to those 
of the corresponding cells of Doto. 
On a preparation preserved in Flemming’s fluid I find many 
of the nucleoli present a different structure from those fixed 
with corrosive sublimate or Kleinenberg’s fluid. Thus many 
of them do not appear homogeneous, but finely granular and 
refractive (Figs. 96 and 97). On the surface of such nucleoli 
occur small, refractive, yellowish globules, which appear black or 
yellow, according to the focus of the microscope; some of them 
are very small. These never occur within the nucleolus, but 
only on its periphery. They may easily be distinguished from 
the chromatin granules by their rounded form and high degree 
of refrangibility, as well as by their deeper yellow color (this 
preparation had been stained with haematoxylin and eosin, but 
the nuclei had not become stained, probably owing to too long 
a fixation in the fluid of Flemming). Numerous other nuclei 
on the same sections showed none of these globules, and none 
were to be seen on preparations which had been differently 
preserved. Accordingly, I consider them to be artefacts. 
