54 
Sarcocystis gazeUae n. sp. 
forms in many instances are distinctly crescentic. Otherwise the above 
description serves very well, but the presence of vacuoles, sometimes 
single, rather large and central, sometimes small and in duplicate, 
should be mentioned. In addition to the two main types of spore, there 
were large oval or irregular shaped forms staining a homogeneous 
blue colour and each containing two separate masses of somewhat 
reticular or granular chromatin, sometimes separated from each other by 
the whole length of the spore and sometimes seen to be side by side. 
These suggested mother cells prior to division into spores, degenerated 
forms, or spores altered in shape by the spreading out of the cyst 
contents on the slide. They were fairly numerous in the smears 
and are shown in figs. 2 and 3. 
In the case of spores stained by the Leishman method the 
chromatin of the polar cap was seen to be distinctly granular. 
A very different appearance, however, was presented when vital 
staining with toluidin blue was employed in the manner I have already 
described (1912). This method quickly differentiated two very distinct 
forms of spore (fig. 6). One was stout, markedly more rounded at one 
end than at the other, took on a dark blue colour, especially at its 
centre, and in nearly all cases exhibited a large vacuole towards the 
more pointed end. This vacuole, which in some spores was very large, 
was not terminal and between it and the sharper end was an area of 
cytoplasm some of which stained as darkly as the central area of the 
spore. The cytoplasm at the blunt end tended to be lighter-coloured. 
The other form was distinctly of a crescent shape, and in many cases 
was very definitely crescentic, possessing pointed ends, one of which 
was, as a rule, a little blunter than the other. The cytoplasm generally 
stained a very pale violet colour, in marked contradistinction to the 
deep blue of the stout spores, and at, or near, the centre were grouped 
violet-coloured granules. In some instances the granules were found 
rather scattered throughout the spore cytoplasm. As regards size 
there did not seem to be much difference between these vitally stained 
forms. The measurements already given apply to them. Some of the 
stout forms were quite as long as the crescentic but looked shorter 
owing to their being thicker. 
It is rather remarkable that it was only by vital staining that the 
above marked difference could be demonstrated. Very little is known 
about the life-history of the sarcosporidia and their study is by no 
means easy, but it is interesting to note that Minchin (1912) says that 
possibly there may be more than one kind of spore even in the same 
