G. H. F. Nuttall 
87 
Copulation was repeatedly observed in vitro. Several males copulated 
with females which had commenced to oviposit. By removing the male 
in some instances the spermatophore was discovered partially introduced 
into the vulva and the parts about the vulva were seen to be bathed 
with secretion. In short the process of copulation takes place as in 
other species of Ixodes. 
Observations relating to oviposition. 
Lot a 
Lot b 
Number of days 
Number of days 
Number of 
which elapsed before 
¥ survived after 
eggs laid 
¥ No. 
oviposition began 
oviposition began* 
by female 
1 
4 
33 
— 
1 
Maintained at an 
2 
3 
28 
— 
- average tempera¬ 
3 
3 
28 
ture of 23° C. 
4 
5 
— 
598 
1 
8 
28 
624 
A 
2 • 
6 
16 
450 
3t 
8 
34 
530 
4 
6 
11 
528 
5+ 
7 
29 
546 
6i' 
5 
19 
602 
7t 
5 
21 
830 
8 
5 
30 
624 
9 
5 
31 
530 
lot 
9 
25 
784 
lit 
5 
13 
594 
12t 
5 
14 
630 
Maintained at an 
13t 
5 
19 
588 
>■ average tempera¬ 
14 
5 
20 
— 
ture of 19° C. 
15 
6 
15 
— 
16t 
9 
33 
— 
17 
5 
38 
— 
18 
5 
26 
— 
19 
5 
40 
— 
20i- 
9 
34 
— 
21 
G 
17 
— 
22 
6 
30 
— 
23t 
5 
24 
— 
24 
5 
27 
— 
25 
4 
26 
— 
> 
* The death of the female usually taking place within 0-6 days of oviposition ceasing, 
t Females observed to copulate on fifth day after collection when placed with males. 
