C. Strickland 
101 
and are seen specially in specimens stained by safranin-methylene-blue 
(see Fig. 10). The myonemes (Fig. 11) are arranged in two layers, one 
superficial, the other deep, the former longitudinal, the latter transverse. 
They are very superficial structures for they may be seen in optical 
section to run right up to the edge of the ectoplasm. Each is distant 
from its neighbour by about 1’7 jx. 
The changes which occur in the structure of the nucleus of the 
sporont are as follows. At first it consists of an oval mass containing a 
closely wound band staining with chromatin dyes. At each pole is 
a moderately large spherical body (Fig. 12) similar to those described 
in the trophozoite. From this form the most striking change is the 
opening out of the chromatin band with the formation of most variable 
Fig. 9. Sporonts. 
Fig. 10. Chromatic granules in the endoplasm of sporont. 
Fig. 11. Anterior end of sporont highly magnified. The transverse myonemes in the 
protomerite are not figured. The longitudinal layer in the protomerite should have 
been figured as going right up to the edge of the parasite in optical section. 
and fantastic figures (see Fig. 13; also Figs. 14, 15); meanwhile the 
chromatin band stains more and more faintly until at last it is no longer 
to be seen. The “polar” bodies also show striking changes. They are 
apparently variable in size and disposition (Fig. 14), and it is possible 
that they reproduce their own kind (Fig. 15). I have found no correlation 
between the unwinding of the chromatin skein and the presence of 
these bodies, but it is very striking how the main features of these 
changes are accompanied by conjugation of the sporonts. 
The cyst. I assume that two sporonts become associated in the gut 
and a cyst wall is formed around them. I have never seen conjugation 
