418 Parasites of the Ground Squirrel 
transitional leucocytes (Figs. 1 and 2). Occasionally they were extra¬ 
cellular (Fig. 3). They were best demonstrated with carbol thionin as 
they were surrounded by a capsular substance which usually resisted 
Wright’s or Jenner’s stains. Prolonged staining with Giemsagave good 
results. In form they are elongated ovoid, sometimes slightly curved, 
with a rather large nucleus which stains uniformly and deeply purple 
with Giemsa after methyl alcohol fixation (Fig. 3), but appears reticu¬ 
lated or granular after heat and carbol thionin (Figs. 1 and 2). The 
Fig. 1. Fig. 2. Fig. 3. 
Fig. 4. 
Fig. 5. 
nucleus is often eccentric. The protoplasm is slightly reticulated and 
filled with basophilic granules after heat and carbol thionin and shows 
fine scattered reddish granules after Giemsa (Fig. 3). Measurements 
gave slight variations, the average being about 16 p, x 6 /x. A few larger 
forms were seen in smears from the lungs of squirrel 2. These were 
extracellular, non-encapsulated, 20 p x 10 p, oval with reticulated proto¬ 
plasm and nucleus composed of isolated masses of chi’omatin (Fig. 4). 
Type in the collection of Wm. B. Wherry. 
Host. This Leucocytozoon was found in two ground squirrels col¬ 
lected in July and August 1909 ; one of these was also infected with a 
trypanosome. 
Squirrel 1. Half-grown female; shot in the Berkeley Hills; ap¬ 
peared normal upon dissection with the exception of a slight congestion 
of the inguinal glands. Leucocytozoa were found, chiefly within the 
polymorphonuclear and transitional cells in smears from the lung, liver, 
spleen and inguinal glands. 
Squirrel 2. Male, about one-third grown ; shot in the Piedmont 
Hills. It appeared to be very ill and made no effort to escape from the 
hunter. Post-mordem the organs showed no noteworthy changes, except¬ 
ing irregular, scattered areas of congestion in the lungs. Smears from 
the lungs showed no bacteria, but numerous motile trypanosomes were 
