430 
Degeneration of T. ganibiense 
the same time as the kineto-niicleus, and trypanosomes may be found 
containing a longitudinal row of granules all dividing at the same time 
to form two rows (figs. 31, 37—39). This appearance can only be 
explained on the assumption of the presence of an axial filament, which 
must usually be achromatic, as it is only occasionally seen. 
In the later stages of degeneration when the axial filament has 
disintegrated, the granules appear irregularly scattered throughout the 
cytoplasm (figs. 42, 43), but they are always concentrated towards the 
anterior extremity (fig. 36). In fact, it seems to be characteristic of all 
the granules, whatever their origin, to move towards the anterior 
extremity of the body. 
The size of the granules is somewhat variable, but as a rule they are 
large when first extruded (fig. 32), and subsequently diminish in size as 
they dissolve in the cytoplasm (fig. 33). When the amount of nuclear 
matter is small the whole of the tropho-nucleus may break down into 
volutinose granules which completely dissolve in the cytoplasm before 
the cell form is lost (fig. 44). Usually, however, the trypanosome breaks 
up into fragments whilst the cytoplasm is still loaded with granules and 
contains a distinct tropho-nucleus, which is often the last structure to 
disappear (figs. 24, 45). These free nuclei bear some resemblance to 
Salvin-Moore and Breinl’s “latent bodies” (1907), but we have not 
found any evidence that they may be regarded as representing anything 
else but the products of degeneration. 
The other kinds of granules, which, for the sake of convenience, have 
been grouped together under the name of chromatic granules, are easily 
distinguished from volutinose, as they stain with iron haematoxylin, as 
Avell as with Giemsa. 
They have two distinct modes of origin, in the one case arising 
from the kineto-nucleus, wdiilst in the other they are derived wholly, 
or in part, from the breaking down of the axial filament. It is 
difficult to distinguish between them, however, as under certain con¬ 
ditions the chromatin from the kineto-nucleus seems to pass along the 
axial filament to form the so-called “black-line,” and when this breaks 
up into granules they usually contain chromatin. 
The particles of chromatin that are given off from the kineto-nucleus 
have been observed in various species of trypanosomes (e.g. Salvin-Moore 
and Breinl, 1908; Kindle, 1909), and Salvin-Moore and Breinl (1908), 
consider the passage of a chromatic body from the kineto-imcleus to the 
region of the tropho-nucleus to have considerable importance in the life 
history of T. equiperdum, regarding it as being homologous with the 
