E. Hindle 
433 
Tlie kineto-vacuole, when present, enlarges considerably and may 
become filled with chromatic substance derived from the kineto-nucleus. 
The axial filament begins to degenerate soon after treatment, often 
breaking up into a row of granules. Occasionally, however, chromatic 
substance from the kineto-nucleus passes along the axial filament, and it 
then appears as a thick black band extending along the middle of the 
cell eventually to the anterior extremity. Subsequently it breaks up 
into granules which dissolve in the cytoplasm of the cell. 
Chromatic bodies are given off from the kineto-nucleus both before 
and after treatment and do not seem to be a characteristic feature of 
degeneration. 
Sometimes the tropho-nucleus completely disappears before the 
cytoplasm breaks up into fragments, but at other times it preserves its 
form long after all other parts of the trypanosome, with the exception 
of the flagellum, have disappeared. These free nuclei resemble the 
“ latent bodies ” described by Salvin-Moore and Breinl, but as they, along 
with the fragments of cytoplasm, granules etc., are ingested by the 
leucocytes, it seems possible that they are merely one of the results of 
degeneration and not part of the life-cycle of this parasite. 
These investigations have been carried on partly with the aid of a 
grant from the Tropical Diseases Research Fund (Colonial Office). 
REFERENCES. 
Hindle, E. (1909). The Life-Histoiy of Trypanosoma diriiorphon. Uiiiv. of Cali¬ 
fornia Puh. ZooL, vol. VI. pp. 127-144. Pis. 15-17, 1 text-figure. 
Minchin, E. a. (1909). The Structure of Trypanosoma lewisi in Relation to 
Microscopic Technique. Quart. Journ. Micr. Sci., vol. Liii. pp. 755-808. 
Pis. 21-23. 
IMinchin, E. a. and Woodcock, H. M. (1910). Observations on certain Blood- 
parasites of Fishes occurring at Rovigno. loc. cit., vol. lv. pp. 113-154. 
Pis. 8-10. 
Nuttall, G. H. F. (1910). The degenerative ajipearances observed in Piroplasma 
canis and Trypanosoma brucei following upon drug treatment. Parasitology, 
vol. III. pp. 203-210. 2 text-figures. 
PoLiCARD A. (1910). Sur la coloration vitale des Trypanosomes. Compt. rend. 
Soc. biol., vol. LXViii. pp. 505-507. 
Robertson, M. (1906). Notes on certain Blood-inhabiting Protozoa. Proc. Phys. 
Soc. Edin., vol. xvi. pp. 232-247. Pis. 8-9. 
Roehl, W. (1909). Heilversuche mit Arsenophenylglycin bei Trypanosomiasis. 
Zeitschr. f. Immunitdts for sell. u. exper. Therapie, vol. i. pp. 033-649. 
