434 
Degeneration of T. gamhiense 
Salvin-Moore, J. E. and Breinl, A. (1907). The Cytology of the Trypanosomes. 
Ann. Trap. Med. Parasitol., vol. i. pp. 441-480. Pis. 38-42. 1 text-figure. 
- (1908). The Life History of Trypanosoma equiperdum. Proc. Roy. Soc., B, 
vol. Lxxx. pp. 288-298. Pis. 8-9. 2 text-figures. 
SwELLEXGREBEL, N. H. (1909). Zur Kenutnis des Banes und der Zellteilung von 
Trypanosoma gamhiense und Tr. equinuni. Tijdschr. d. Ned. Dierk. Vereen (2), 
vol. XI. pp. 80-98. PI. 5. 
Woodcock, H. M. (1906). The Haemoflagellates : A review of present knowledge 
relating to the trypanosomes and allied forms. Part I. Quart. Journ. Micr. 
Soi, vol. L. pp. 151-231. 22 text-figures. 
EXPLANATION OF PLATE XXX. 
All the figures are drawn to a magnification of 2400 diameters with the exception of 
Figs. 17—23 which are magnified 4000 diameters. 
Figs. 1—25. Trypanosoma gamhiense, drawn from films stained with iron-haematoxylin. 
Fig. 1. Indifferent form, 1 hr. after treatment, shewing rounded posterior extremity. 
Fig. 2. Similar form shewing a small kineto-vacuole and chromatic granules between the 
two nuclei. 
Fig. 3. Trypanosome, 1 hr. after treatment, shewing a trace of the axial filament between 
the kineto-vacuole and trophdinu'cleus, the latter containing granules of volutinose. 
Fig. 4. Ordinary dividing form, 1 hr. after treatment. 
Fig. 5. Dividing trypanosome, 2 hrs. after treatment, shewing a black line extending 
down from the kineto-vacuole. 
Fig. 6. Trypanosome, 1 hr. after treatment, shewing a dividing granule between the two 
nuclei. 
Fig. 7. Trypanosome, 2 hrs. after treatment, containing a spiral band of chromatin in 
place of the tropho-nucleus, and partly developed axial filament. 
Figs. 8—10. Various Degeneration stages of the axial filament. (2 hrs. after treatment.) 
Fig. 11. Small form, 3 hrs. after treatment, containing a well-developed “ black line” 
extending to the anterior extremity. 
Fig. 12. Advanced stage of degeneration, 3 hrs. after treatment. The kineto-vacuole is 
filled with chromatic substance and the tropho-nucleus has broken up into fragments. 
Fig. 13. Trypanosome, 2 hrs. after treatment, shewing a deeply stained axial filament 
(black line) ending about the region of the tropho-nucleus. 
Fig. 14. Drawn from the same film as the preceding figure. 
Fig. 15. Large dividing (?) form, 3 hrs. after treatment, with a well developed axial 
filament arising from a large kineto-vacuole. 
Fig. 16. Male form, 3 hrs. after treatment. 
Figs. 17—23. Various tropho-nuclei shewing the increase in the number of granules 
(X 4000). 
Fig. 24. Free tropho-nucleus and flagellum, 5 hrs. after treatment. The rest of the 
parasite has disappeared. 
Fig. 25. “Bounded up ” form, 4 hrs. after treatment. 
Figs. 26—44. Drawn from dry films stained with Giemsa, except when otherwise stated. 
Fig. 26. Indifferent type of trypanosome before treatment. (Std. Azur.) 
